Nowadays, the application of glass nanopipettes in electrochemical analysis has attracted a lot of attention with the increasing demand for sensitive analysis and diagnostic tools for cellular biomolecules. The ultrasmall nanopipettes are becoming a bridge between the macro-and nanoworlds as they can transport very small volumes of liquids and materials as well as acting as electrochemical biosensors. In this minireview, we discuss the fabrication and characterization of nanopipettes and introduce our recent studies using nanopipettes for cell electroanalysis. The future application of nanopipettes has also been discussed.

Nanopipette, which is fabricated by glasses and possesses a nanoscale pore in the tip, has been proven to be immensely useful in electrochemical analysis. Numerous nanopipette‐based sensors have emerged with improved sensitivity, selectivity, ease of use, and miniaturization. In this minireview, we provide an overview of the recent developments of nanopipette‐based electrochemical sensors based on different types of nanopipettes, including single‐nanopipettes, self‐referenced nanopipettes, dual‐nanopipettes, and double‐barrel nanopipettes. Several important modification materials for nanopipette functionalization are highlighted, such as conductive materials, macromolecular materials, and functional molecules. These materials can improve the sensing performance and targeting specificities of nanopipettes. We also discuss examples of related applications and the future development of nanopipette‐based strategies.