Abstract. In order to evaluate the sugar content and composition of 'Angeleno' plum, a method for the simultaneous determination of fructose, glucose, sucrose, sorbitol in Angeleno fruit was developed with HPLC-ELSD .The analysis was performed on an Agilent Zorbax column with a gradient program, acetonitrile-water as the mobile phase . The column temperature was 35 ℃ and flow rate was 1.40 ml/min, evaporative light scattering detector, the drift tube temperature was 80 ℃. The Results showed that 'Angeleno' plum fruit mainly contain fructose, sorbitol, glucose and sucrose , four kinds of sweet substances, the average recoveries in the 92.08% ~101.31%; RSD in the range of 0.52% ~ 3.68%. In a conclusion, the method was accuracy, sensitivity and feasibility to analyze the four kinds of soluble sugar in Angeleno plum fruit.
Scanning electron microscopy was used to line scan 7 positions (pronotum, scutellum, elytra, abdominal sternites, head, maxillary palpus and labial palpus) of 11 Harmonia axyridis Pallas with different color patterns to make up for the lack of general morphological observation, and at the same time it was also used to further study the relationship of ultrastructure and predatory behavior of Harmonia axyridis Pallas to establish the important research foundation. The results show that the hair on the body of the elytra of the different H. axyridis directly stretches out from the surface of the hole and only few hairs on the body of the elytra stretched out from the concave surface of the elytra. Moreover, the hollow on the elytra edge increased significantly, though no two body hairs were produced on one hollow, and the granular material was borne on the first baseline web splices. It was observed that the hair densities of pronotum, scutellum, elytra, and abdominal sternites from the 11 types of H. axyridis Pallas showed some diversity. Likewise, the number of sensilla from the head, abdominal sternites, maxillary palpus, and labial palpus showed some differences. These findings highlight the fact that ultrastructure supplement was provided to the morphological aspect of H. axyridis.
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