Silver(i) chalcogenide/chalcogenolate clusters are promising photofunctional materials for sensing, optoelectronics and solar energy harvesting applications. However, their instability and poor room-temperature luminescent quantum yields have hampered more extensive study. Here, we graft such clusters to adaptable bridging ligands, enabling their interconnection and the formation of rigid metal-organic frameworks. By controlling the spatial separation and orientation of the clusters, they then exhibit enhanced stability (over one year) and quantum yield (12.1%). Ultrafast dual-function fluorescence switching (<1 s) is also achieved, with turn-off triggered by O and multicoloured turn-on by volatile organic compounds. Single-crystal X-ray diffraction of the inclusion materials, obtained by single-crystal-to-single-crystal transformation, enables precise determination of the position of the small molecules within the framework, elucidating the switching mechanism. The work enriches the cluster-based metal-organic framework portfolio, bridges the gap between silver chalcogenide/chalcogenolate clusters and metal-organic frameworks, and provides a foundation for further development of functional silver-cluster-based materials.

Honeycomb poly(ε-caprolactone) (PCL) films with tunable pore diameters of 3.5, 6.0, and 10 μm were fabricated directly from solutions in water-miscible, relatively nontoxic tetrahydrofuran using the breath-figure method without assistance of a surfactant. These honeycomb PCL films were characterized in terms of structures and enhanced hydrophobicity. Aiming at fostering bone tissue engineering outcomes, we cultured mouse preosteoblastic MC3T3-E1 cells on these honeycomb films as well as on the flat control, and evaluated their adhesion, spreading, proliferation, alkaline phosphatase (ALP) activity, and calcium content. These cell behaviors were further correlated with the expression levels of integrin subunits of α(1), α(2), β(1), and bone-specific gene markers of ALP, collagen type I (COL I), osteocalcin (OCN), and osteopontin (OPN). Honeycomb PCL films remarkably promoted MC3T3-E1 cell adhesion, spreading, proliferation, differentiation, and gene expression. This effect was more prominent when the pore diameter was smaller in the studied range. In addition, honeycomb PCL films were stretched into groove-like structures, on which MC3T3-E1 cells were aligned with a smaller cell area, a higher percentage of aligned cells, and a higher cell elongation ratio when the pores were smaller.

Extradomain-B fibronectin (EDB-FN), one of the oncofetal fibronectin (onfFN) isoforms, is a high-molecular-weight glycoprotein that mediates cell adhesion and migration. The expression of EDB-FN is associated with a number of cancer-related biological processes such as tumorigenesis, angiogenesis, and epithelial-to-mesenchymal transition (EMT). Here, we report the development of a small peptide specific to EDB-FN for targeting prostate cancer. A cyclic nonapeptide, CTVRTSADC (ZD2), was identified using peptide phage display. A ZD2-Cy5 conjugate was synthesized to accomplish molecular imaging of prostate cancer in vitro and in vivo. ZD2-Cy5 demonstrated effective binding to up-regulated EDB-FN secreted by TGF-β-induced PC3 cancer cells following EMT. Following intravenous injections, the targeted fluorescent probe specifically bound to and delineated PC3-GFP prostate tumors in nude mice bearing the tumor xenografts. ZD2-Cy5 also showed stronger binding to human prostate tumor specimens with a higher Gleason score (GS9) compared to those with a lower score (GS 7), with no binding in benign prostatic hyperplasia (BPH). Thus, the ZD2 peptide is a promising strategy for molecular imaging and targeted therapy of prostate cancer.

ABSTRACT:In this study, electrospinning was used to fabricate ethyl-cyanoethyl cellulose [(E-CE)C] fiber from a solution of (E-CE)C/tetrahydrofuran. The diameter of the thinnest fiber fabricated during the electrospinning was about 200 nm. It was found that the diameters of the fibers and their distribution depend on the processing parameters and properties of the solution, such as viscosity, temperature, and concentration, for example. The morphology of the fiber was also observed by SEM.