The signal‐to‐noise ratio (SNR) is one of the key features of a fluorescent probe and one that often defines its potential utility for in vivo labeling and analyte detection applications. Here, it is reported that introducing a pyridine group into traditional cyanine‐7 dyes in an asymmetric manner provides a series of tunable NIR fluorescent dyes (Cy‐Mu‐7) characterized by enhanced Stokes shifts (≈230 nm) compared to the parent cyanine 7 dye (<25 nm). The observed Stokes shift increase is ascribed to symmetry breaking of the Cy‐Mu‐7 core and a reduction in the extent of conjugation. The fluorescence signals of the Cy‐Mu‐7 dyes are enhanced upon confinement within the hydrophobic cavity of albumin or via spontaneous encapsulation within micelles in aqueous media. Utilizing the Cy‐Mu‐7, ultra‐fast in vivo kidney labeling in mice is realized, and it is found that the liver injury will aggravate the burden of kidney by monitoring the fluorescence intensity ratio of kidney to liver. In addition, Cy‐Mu‐7 could be used as efficient chemiluminescence resonance energy transfer acceptor for the reaction between H 2 O 2 and bisoxalate. The potential utility of Cy‐Mu‐7 is illustrated via direct monitoring fluctuations in endogenous H 2 O 2 levels in a mouse model to mimic emergency room trauma.

Aiming at the flatness difference between thick strip and thin strip under the same flatness control strategy on a 2250 mm hot tandem rolling line, a flatness calculation model is firstly established in this paper. With the unknown variable–transversal flow of metal solved by finite element methods, the flatness calculation model is used to analyze and compare the flatness and longitudinal residual stress evolution during thick strip and thin strip rolling. The result shows that under the premise of the constant exit relative crown in the downstream stands, the distribution of metal transversal flow due to larger reduction ratio in F2 and F3 stand causes the flatness of thin strip differing much from that of thick strip. In order to improve the flatness quality, a crown setup method is proposed. Based on the relationship among the distribution of metal transversal flow, entry relative crown, and the change of relative crown obtained from the finite element model, the exit crown of thin strip at each stand is optimized by genetic algorithm with a restriction of achieving the target crown at exit of F7 stand and a goal of good flatness quality during rolling.