Introduction: RNA-based therapy for bone repair and regeneration is a highly safe and effective approach, which has been extensively investigated in recent years. However, the molecular stability of RNA agents still remains insufficient for clinical application. High porosity, tunable size, and ideal biodegradability and biosafety are a few of the characters of mesoporous silicon nanoparticles (MSNs) that render them a promising biomaterial carrier for RNA treatment. Materials and Methods: In this study, a novel miR-26a delivery system was constructed based on MSNs. Next, we assessed the miRNA protection of the delivery vehicles. Then, rat bone marrow mesenchymal stem cells (rBMSCs) were incubated with the vectors, and the transfection efficiency, cellular uptake, and effects on cell viability and osteogenic differentiation were evaluated. Results: The results demonstrated that the vectors protected miR-26a from degradation in vitro and delivered it into the cytoplasm. A relatively low concentration of the delivery systems significantly increased osteogenic differentiation of rBMSCs. Conclusion: The vectors constructed in our study provide new methods and strategies for the delivery of microRNAs in bone tissue engineering.

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We aimed to investigate the influence of different fiberglass surface pretreatments on the interfacial and mechanical properties of fiberglass-reinforced photocurable (FRP) resin pile and evaluate their cytotoxicity. The fiberglass was treated by different types, such as heat and acid treatment, and then modified by KH570, which were immersed in a laboratory-prepared photocurable resin substrate to prepare FRP resin piles. Scanning electron microscope (SEM), thermogravimetric analyses (TGA) and Fourier transform infrared spectrum (FT-IR) were used to characterize the morphologies and structures.Furthermore, the flexural modulus, bending strength, and bending load of the FRP resin piles were analyzed and the cytotoxicity on L929 cells were measured via methyl thiazol tetrazolium (MTT) assay.The results showed that high treatment could enhance the combination of the fiberglass with KH570 and then mechanical properties of the FRP resin piles, but acid treatment could reduce the performance of the FRP resin piles. MTT assay revealed the low cytotoxicity, which could be a potential application.

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