Several factors affecting the frequency of leaf regeneration of Ziziphus jujuba 'Huizao' were investigated in this study, including basal medium, plant growth regulators, leaf maturity, explant orientation, and additive chemicals. The results showed that the highest shoot regeneration frequency (82.25%) was obtained on woody plant medium supplemented with 2.27 lM thidiazuron, 1.07 lM a-naphthalene-acetic acid (NAA) and 2.94 lM silver nitrate after a 10 days incubation in darkness. This study also suggested an increased regeneration frequency of expanded young leaves which were taken from the midstem position, as well as from the explant abaxial surface contacting with the medium. In addition, hyperhydric of adventitious buds could be effectively reduced by adding 40 g l -1 sucrose to the medium. A 95.56% rooting rate could be produced from shoots cultured on half-strength Murashige and Skoog (in Physiol Plant 15:473-497, 1962) medium plus 2.69 lM NAA.