This study discussed Astragalus Polysaccharides (APS)’s effect on the cytobiology of glioma. U87 glioma cells were assigned into control group (U87 cells), miR-34a-5p mimic group (transfected with miR-34a-5p mimic), and APS group (treated with 10 μM APS) followed by analysis of miR-34a-5p level, cell proliferation and invasion, Caspase3 and SOD activity as well as E-cadherin, Vimentin and survivn expression. APS treatment significantly upregulated miR-34a-5p expression, inhibits cell proliferation and invasion, and promoted cell apoptosis. In addition, APS also significantly upregulated E-cadherin, downregulated Vimentin and survivn level in glioma cells as well as inhibited ROS generation and increased SOD activity. In conclusion, the level of miR-34a-5a in glioma cells is up-regulated by APS so as to restrain the biological behaviors of glioma cells, indicating that it might be used as novel agent for the treatment of glioma.

Our study aims to discuss the effect of retinoid drug on autophagy of medulloblastoma cells. Targeted ferrocenoretinoic acid was prepared and identified. The MB cells were assigned into blank group, control group and transfection group followed by analysis of cell survival rate and expression of Rack1, Hedgehog-Gli, Beclin1 and LC3. The size and form of prepared ferrocenoretinoic acid was uniform. There was positive charge which can bind target. Ferrocenoretinoic acid treatment declined cell survival rate and increased cell apoptotic rate. The level of Rack1 and Hedgehog-Gli in transfection group was lower than other two group. The tendency in expression of Beclin1 and LC3 was reversed. In conclusion, the expression of Rack1 is restrained by nano-retinoid drug so as to restrain the Hedgehog-Gli signal activity. Therefore, the survival rate of medulloblastoma cells could be restrained and apoptotic rate could be prompted.