Recently, following the rapid development of information technology and computer technology, various universities have higher and higher requirements for the management efficiency of their laboratories. Therefore, this paper studies the laboratory operation and maintenance management system (LOMMS) based on video surveillance, focusing on the detection and tracking of moving targets in the laboratory, and detecting possible abnormal behaviors. Compared with the traditional manual operation and maintenance management laboratory, the efficiency of LOMMS is significantly improved. At the same time, LOMSS video surveillance ensures the normal operation of the laboratory and the safety of the laboratory. This paper designs the complete scheme of LOMSS comprehensively with the video surveillance technology and the multisensor technology. First of all, through the detailed demand analysis of the university laboratory related staff, teachers, and students, the research objectives of LOMSS based on video surveillance are determined through repeated investigation with them. Secondly, through the demand analysis results, combined with the characteristics of LOMSS based on video surveillance, the system function is generally designed. Thirdly, based on the top-down principle, the overall function of the designed system is differentiated to obtain a more detailed functional map. At the same time, the database of LOMSS is designed to better store the data and other information in the system. The experimental results show that the temperature collected by the sensor is 10∼30°C, and the humidity is 35∼65%, which meets the environmental standards of the laboratory. This also shows that the test results of the sensor are normal, and also shows that the intelligent monitoring operation and maintenance management system (IMOMMS) of the laboratory meets the needs of the laboratory.