Background Polycystic ovary syndrome (PCOS) is a complex endocrine disease that is one of the most common disease of reproductive age and is one of the important factors leading to infertility in young women, but the potential molecular mechanism remains unclear. In this study, we identified previously unrecognized m7G-related and cuproptosis-related genes that are significantly correlated with PCOS via bioinformatics. Materials and methods Three transcriptome profiles of granulosa cells of PCOS sample and normal samples were obtained from online databases. Comprehensive bioinformatic analyses, such as differential expression analysis, functional and pathway enrichment analysis, immune infiltration analysis, were utilized. Results Three PCOS datasets (GSE34526, GSE80432, and GSE137684) from the Gene Expression Omnibus (GEO) database were integrated and the background correction and normalization were performed by R language. The corrected gene cuproptosis-related genes and m7G-related genes respectively to obtain intersection genes. There were 12 intersection genes relate to cuproptosis-related genes and 21 intersection genes relate to m7G-related genes in comparison between PCOS group and normal group. After the correction gene crossed with the immune infiltrating gene, it crossed with 12 intersection genes relate to cuproptosis-related genes and 21 intersection genes relate to m7G-related genes respectively. Ultimately, it was identified that 8 genes related to cuproptosis are related to immune infiltration (CIGs), among which DLAT was the most significant (P = 0.049); 5 m7G related genes are associated with immune infiltration (MIGs), among which NUDT16 was the most significant (P = 0.0246). There were significant differences in clinicopathological characteristics between the PCOS group and the normal group by ROC curve analysis. Conclusions This study addresses the information gap in PCOS research about the interaction between immunology, m7G, and copper metabolism. According to the PCOS prediction model, the genes m7G and those associated to copper metabolism are crucial in the onset and progression of the condition. This study has further explored the microenvironment of ovarian granulosa cell of PCOS development and immune factor related genes. This work will support future research.

Background. Recurrent implantation failure (RIF), a clinical disorder characterized by failure to achieve pregnancy after repeated (≥3) embryo transfer, is a challenge for reproductive demands worldwide. In our preliminary work, the Zhuyun formula (ZYF) with auricular acupressure, a complementary and alternative medicine (CAM) with a small sample size for RIF, can improve the clinical pregnancy rate (41.2% vs. 26.7%, treatment group vs. control group, p < 0.05 ). Based on the toxicological and pregnancy-related pharmacological analysis of ZYF for RIF, the T-cell receptor signaling pathway might be involved in the pharmacological activity. This study aimed at evaluating the efficacy and safety of the CAM therapy according to pregnancy outcomes and maternal and child health and investigating the changes of T-helper (Th) cells in the peripheral blood of unexplained RIF women. Materials and Methods. We conducted a prospective, two-arms, randomized, nonblinded study. All eligible women were randomly assigned to the treatment group (TG) and the control group (CG) according to a computer-generated randomization list in sealed opaque envelopes. Blood samples were collected from the two groups, and serum Th1, Th2, and Treg were detected by flow cytometry. The cytokines were detected by an enzyme-linked immunosorbent assay (ELISA). The TG was administrated with ZYF and auricular acupressure for three months before ovarian stimulation, while the control group was on a waiting list for the same period. The primary outcome was CPR. The second outcomes were the serum levels of immune parameters. For the safety evaluation, the perinatal outcomes of maternal and child were obtained by follow-up. Post-hoc sensitivity analyses were performed to assess the effect of missing data. Results. One hundred and twenty-three women were randomized into the TG (n = 62) and CG (n = 61). The CPR was increased significantly in the TG (45.2%) than CG (26.2%) (p = 0.029). Twenty blood samples were collected, and the Th2/Th1 and Treg expression level was significantly higher in the TG than in the CG. IL-2, IL-10, and Foxp3 were higher significantly in the TG than in the CG. The maternal and child perinatal outcomes were not significantly different between the two groups. Conclusions. The ZYF with auricular acupressure was effective and safe in improving the pregnancy outcomes of RIF. It might be related to balancing the level of cytokines related to the immune tolerance of the maternal-fetal interface to protect the embryo from the maternal immune system. Clinical Trial Registration: Clinical Trial Registry; date: 14/Dec/2013; no. NCT03078205.