Gentiana macrophylla Pall. (G. macrophylla)—a member of the family Gentianaceae—is a well-known traditional Chinese medical herb. Iridoids are the main active components of G. macrophylla, which has a wide range of pharmacological activities such as dispelling wind, eliminating dampness, clearing heat and asthenic fever, hepatoprotective and choleretic actions, and other medicinal effects. In this study, a total of 67,048 unigenes were obtained by transcriptomic sequencing analysis of G. macrophylla. A BLAST analysis showed that 48.21%, 33.66%, 46.32%, and 32.62% of unigenes were identified in the NR, Swiss-Prot, eggNOG, and KEGG databases, respectively. Twenty-five key enzymes were identified in the iridoid biosynthesis pathway. Most of the upregulated unigenes were enriched in flowers and leaves. The trustworthiness of the transcriptomic data was validated by real-time quantitative PCR (qRT-PCR). A total of 22 chemical constituents were identified by ultra-high performance liquid chromatography-quadrupole-electrostatic field Orbitrap mass spectrometry (UPLC-Q-Exactive MS), including 10 iridoids. A correlation analysis showed that the expression of 7-DLH and SLS was closely related to iridoids. The expression of 7-DLH and SLS was higher in flowers, indicating that flowers are important for iridoid biosynthesis in G. macrophylla.

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Objective To explore the status of undergraduates’ WeChat usage and upward social comparison, and analyze the relationship between the above two variables.Method：Totally 754 WeChat undergraduates were selected through poster recruitment from 5 colleges in Guangdong Province. They were investigated with Access Frequency to Social Networking Site Scale (AFSNSS), Upward Social Comparison Subscale of Iowa-Nwtherlands Comparison Orientation Measure (INCOM-USCS). Results：The score of overall emotional engagement(OEE) of AFSNSS and the total standard score of INCOM–USCS (TSSI-U) were (22.41±4.70) and (0±4.5), respectively. The correlation between WeChat frequency and TSSI-U was not significant.TSSI-U was significantly postively correlated with the score of OEE and other items of AFSNSS (r=0.161～0.413, P <0.01). Multiple linear regression analysis showed that The scores of item 1,7and 8 of AFSNSS, as well as family economic status, grade ranking of academic performance (GRAP) and the purpose of WeChat use were postively correlated with TSSI-U (β=.104～.234, P<.05). Class cadre or not and origin were negatively correlated with TSSI-U (β=-.089, -.130,P<.05). Conclusion：It suggests that WeChat usage may be a related factor for undergraduates'upward social comparison.

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