BackgroundAntiangiogenic therapy is one of the most significant advances in anticancer treatment. The benefits of antiangiogenic therapies of late-stage cancers have been investigated but are still too limited.MethodsWe used an ovarian cancer model to test the effect of short-term bevacizumab treatment on metastasis as measured by bioluminescence. Western blotting and CD34-PAS dual staining were performed to assess hypoxia-inducible transcription factor-1α (HIF-1α) expression and vasculogenic mimicry(VM) formation. Cell viability was examined by a CCK8 assay.ResultsBevacizumab demonstrated antitumor effects in models of ovarian cancer, but also accelerated metastasis together, with marked hypoxia and VM formation in mice receiving short-term therapy. Bevacizumab treatment did not affect SKOV3 cell viability and the amount of VM in three-dimensional culture.ConclusionThese results suggest that antiangiogenic therapy may potentially influence the progression of metastatic disease, which has been linked to the hypoxic response and VM formation.
DNA-encoded library
(DEL) is an efficient high-throughput screening
technology platform in drug discovery and is also gaining momentum
in academic research. Today, the majority of DELs are assembled and
encoded with double-stranded DNA tags (dsDELs) and has been selected
against numerous biological targets; however, dsDELs are not amendable
to some of the recently developed selection methods, such as the cross-linking-based
selection against immobilized targets and live-cell-based selections,
which require DELs encoded with single-stranded DNAs (ssDELs). Herein,
we present a simple method to convert dsDELs to ssDELs using exonuclease
digestion without library redesign and resynthesis. We show that dsDELs
could be efficiently converted to ssDELs and used for affinity-based
selections either with purified proteins or on live cells.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.