Integration of disease diagnosis and therapy in vivo by nanotechnology is a challenge in the design of multifunctional nanocarriers. Herein, we report an intelligent and degradable nanoreactor, an assembly of the 4-mercaptobenzonitrile-decorated silver nanoparticles (AgNPs@MBN) and the glucose oxidase (GOx)-loaded metal-organic-framework (ZIF-8@GOx), which can be activated by tumor microenvironment to start the catalytic cascade-enhanced chemo-starvation synergistic therapy and simultaneous self-sense of cellular glucose level. Under the mild acidic microenvironment of tumor, the nanoreactor will collapse to release GOx that triggers a catalytic cascade reaction in vivo, depleting glucose, etching AgNPs@MBN, and producing toxic H 2 O 2 , Ag + , and Zn 2+ ions, all of which work together to inhibit tumor growth. The AgNPs@MBN as SERS nanoprobe reads out glucose concentration noninvasively in tumor to achieve instant feedback of therapeutic progression. This work proposes a promising example of using enzyme-encapsulated biomineralized MOFs as an effective anticarcinogen for clinical applications.Recently, glucose oxidase (GOx)-based cancer starvation therapy that depletes glucose and engenders toxic H 2 O 2 /gluconic acid in the presence of oxygen has been explored (Chang et al., 2017;Wang et al., 2016; Zhang et al., 2018a) as a targeting therapeutic strategy for cancers due to its powerful ability to change tumor microenvironments. Moreover, the generated H 2 O 2 not only markedly enhances tumor oxidative stress but also can be converted into $OH radicals to kill cancer cells (Wang et al., 2019b). Thus this strategy can be further integrated with other therapeutic methods to achieve enhanced synergistic therapeutic effects (Wang et al., 2019c;Ma et al., 2019;Tang et al., 2019). The naked GOx exposed in biological environments is particularly prone to inactivation. Also, it has the weaknesses of short in vivo half-life, immunogenicity, and systematic toxicity (Fu et al., 2018). Thus, nanocarriers (inorganic or organic) for GOx (Wang et al., 2016; Zhang et al., 2018a) are highly suggested in many GOx-based starvation therapeutic strategies. However, these nanocarriers are usually undegradable in vivo, and hence may cause biotoxicity. Therefore, a degradable and intelligent nanocarrier for precise GOx-based starvation therapy is urgently desired.Metal-organic frameworks (MOFs) are a class of highly crystalline, porous, and degradable solid-state materials constructed by metal ions and organic linkers (Wang et al., 2019a;Schoedel et al., 2016). It affords a promising biomedical nanocarrier platform for encapsulating drugs, antibodies, genes, enzymes, etc. (Feng et al., 2018;Chen et al., 2019) for in vivo drug delivery. These MOF cages can protect proteins from the attack of proteases and the clearance of the mononuclear phagocyte system in physiological environments (
