Guanine nucleotide-binding protein subunit α14 (GNA14) knockdown was demonstrated to inhibit the proliferation of endometrial carcinoma cells in a recent study; however, its role in hepatocellular carcinoma (HCC) is unknown. In the present study, the clinical significance of GNA14 in HCC was assessed using a dataset of patients with HCC from The Cancer Genome Atlas database. The Integrative Molecular Database of Hepatocellular Carcinoma and Oncomine databases were also used to identify the expression levels of GNA14 in HCC tissues. The association between GNA14 expression levels and clinicopathological features was assessed using the Wilcoxon signed-rank test and logistic regression analysis. Kaplan-Meier curves and Cox regression analysis were applied to evaluate the independent risk factors for clinical outcomes. The present study determined GNA14 DNA methylation levels and tumor-infiltrating immune cells, as well as used Gene Set Enrichment Analysis (GSEA) in HCC. GNA14 mRNA expression levels were lower in HCC compared with normal tissues. Downregulation of GNA14 in HCC was significantly associated with tumor grade, clinical stage and T stage. Furthermore, low expression of GNA14 was an independent predictor for survival outcomes. GNA14 expression levels were partially correlated with the infiltration of B cells and macrophages. Additionally, GSEA analysis revealed that the expression levels of GNA14 were associated with multiple signaling pathways, such as translation, DNA replication, and homologous recombination.In conclusion, low GNA14 expression may be a novel biomarker for diagnosis and prognosis prediction for patients with HCC.

A urinary bladder stone in young adults is uncommon. Dislocation of an IUD to adjection organs is a rare condition. We present a case of a 28-year female with a chief complaint of right side pelvis discomfort, off and on with the urinary system. In this case, we performed cystoscopy assisted laser lithotripsy, hysteroscopy to localize and remove IUD, transurethral resectoscope for removing IUD residual, and resection sinus tract. This article's objective states that the multidisciplinary approach to removing dislocated IUD is safe and effective and raises awareness of forgotten contraceptive devices and their potential complications.

Objective: To investigate the impact of chlorhexidine acetate's on the mucus secretion function in the early postoperative period of the neo-ileal bladder and compare other treatment agents. Method: 24 patients who underwent total Cystectomy with orthotopic ileal neobladder in our department and divided into three groups (8 each). During the procedure, mucosal treatment agents were infused for five minutes, and specimens were taken for histological examination of the neobladder mucosa under light and electron microscopy.Results: The daily intestinal mucus secretion in the early stage of the neobladder(POD 3-14). Group A (Iodophor) compared to B (anhydrous ethanol), and C (chlorhexidine acetate) were reduced and statistically significant (P <0.05), with no statistical difference between B and C (P> 0.05). Light and Electron microscopy; In group A, the neobladder intestinal mucosa layer is complete; villi were uniformly arranged. A few goblet cells between the apex of the villi disappear an acceptable arrangement of micro-villi and a relatively low degree of organelle damage. In group B, the neobladder intestinal mucosa layer is unclear, the villi arrangement lost and shedding, muscle layer structure disrupted, accompanied by RBC and inflammatory cells infiltration. In group C, neo-bladder intestinal mucosa structure was complete, and villi are low, rupture, shedding, and inflammatory cells infiltrated without significant damage muscle layer.Conclusion: As a new mucous membrane treatment agent, chlorhexidine acetate has a protective effect on the mucous membrane of the new bladder. Meanwhile, it also inhibits the secretion of mucous in the new bladder in the early postoperative period.