The phosphatase-like activity of ZrO2 nanoparticles is reported, which has found applications in intracellular imaging.

The alkaline phosphatase-like nanozyme was applied for immunoassay for the first time. By using nanoceria as the alkaline phosphatase-like catalytic label and CDP-star as the substrate, the chemiluminescent detection of...

The phosphatase-like activity of Ce­(IV) ions was applied for chemiluminescence (CL) analysis for the first time. Ce­(IV) can catalyze the hydrolysis of CDP-star, which is a phosphatase substrate, to produce strong CL emission. The CL performance of the Ce­(IV)/CDP-star system can be significantly improved by the addition of ionic liquids. In the presence of 1-butyl-3-methylimidazolium tetrafluoroborate, the selective and sensitive CL detection of Ce­(IV) ions was achieved with a detection limit of 460 nM. The proposed CL system was also used for the detection of ascorbic acid and ClO–. It is based on the phenomenon that Ce­(IV) can catalyze the hydrolysis of CDP-star, while Ce­(III) cannot. The introduction of reductive ascorbic acid into the mixture of Ce­(IV)/CDP-star can turn off the CL signal, while the addition of oxidative ClO– into the solution of Ce­(III)/CDP-star can turn on the CL emission. Finally, Ce­(IV)/CDP-star CL was successfully applied for evaluating the total antioxidant capacity in commercial fruit juice samples.