In order to revealed the spatial-temporal correlation characteristics and interaction relationship of multifunctional land use in the county of Jiangsu Province, which could provide theoretical support for the spatial planning of the county. This paper constructed multiple functional identification system from three dimensions of production-living-ecological, and quantitatively measures 55 counties' spatial equilibrium state and space-time effect of "production-living-ecological" function in 1996, 2005 and 2015 by means of coupling coordination degree model, Theil coefficient and its decomposition, and LISA time path analysis. The results showed that spatial equilibrium level of multifunctional land use function in the county of Jiangsu Province was relatively low from 1996 to 2015, presenting the spatial characteristics of the overall dispersion and local agglomeration. The coordination degree of multifunctional land use had spatial differentiation characteristics,which were closely related to the stage of social economic development. The time-space transition of multiple functional coordination degree was generally consistent with the cold-hot spot pattern. The regions with relatively long LISA time path were scattered in southern Jiangsu (Nanjing, Suzhou, Wuxi, Changzhou and Yangzhou). The regions with relatively short LISA time path were scattered in northern Jiangsu, central Jiangsu. The multiple functional coordination degree was relatively low in these areas.The county with the largest LISA time path curvature were Zhenjiang, Xinghua, Yancheng, Guanyun etc. Nevertheless, the curvature of LISA time path in most parts of central Jiangsu and northern Jiangsu was generally small. It can be seen that the path locking effect of development of multifunctional land use coordination degree was strong.

The Ag85 complex functions as the main secretory protein of Mycobacterium tuberculosis (M. tuberculosis) and BCG. This complex is composed of the proteins, Ag85A, Ag85B, and Ag85C, with Ag85A thought to play the largest role within the complex. However, the lack of commercially available monoclonal antibodies (mAbs) against Ag85A still hinders the biological and applicative research on this protein. In this study, we developed and identified anti-Ag85A mAbs, and five hybridoma cells were established. Using the indirect immunofluorescence test, we found that two anti-Ag85A mAbs did not cross-react with Ag85B and/or Ag85C. In addition, we showed that all of the mAbs tested in this study are able to react with endogenous Ag85A protein in BCG and rBCG:Ag85A using indirect ELISA and Western blot analyses. A competitive ELISA (cELISA) based on mAb 3B8 was developed, the analyses of clinic serum samples from cattle with bovine tuberculosis (TB) and healthy cattle demonstrated that the sensitivity of the cELISA was 54.2% (26/48) and the specificity was 83.5% (167/200). This study demonstrated that the mAbs against Ag85A will provide useful reagents for further investigation into the function of the Ag85 complex and can be used for serodiagnosis of bovine TB.

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The dislocated development of population, land, and economy will disturb the urban system, cause ecological risk problems, and ultimately affect regional habitat and quality development. Based on social statistics and nighttime lighting data from 2000 to 2018, we used mathematical statistics and spatial analysis methods to analyze the change process of urbanization’s coupling coordination degree and ecological risk response pattern in the Yangtze River Delta. Results show that: ① From 2000 to 2018, the coupling coordination degree of urbanization in the Yangtze River Delta increased, with high values in Suzhou-Wuxi-Changzhou, Shanghai, Nanjing and Hangzhou regions. ② The ecological risk in the Yangtze River Delta weakened, and the vulnerability and disturbance of landscape components together constitute the spatial differentiation pattern of regional ecological risk, which presented homogeneous aggregation and heterogeneous isolation. ③ The overall ecological stress of urbanization in the Yangtze River Delta decreased. ④ The population aggregation degree, socio-economic development level and built-up area expansion trend contributed to the spatiotemporal differentiation of urbanization’s ecological risks through the synergistic effects of factor concentration and diffusion, population quality cultivation and improvement, technological progress and dispersion, industrial structure adjustment and upgrading. This study can provide a reference for regional urbanization to deal with ecological risks reasonably and achieve high-quality development.

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