For the micro-milling of hard and brittle materials, to avoid crack formation, a tool with ductile milling mode is required. Composite electrodeposition technology was used to prepare a Ni–diamond coating on the surface of brass. The surface microstructure, composition and surface roughness of the coating were studied with a scanning electron microscope, X-ray diffractometer and roughness tester. The adhesion strength was studied by scratch test, the wear resistance was analyzed by wear test, and the corrosion resistance was investigated by Tafel curves and electrochemical impedance spectra (EIS). It was found that the distribution of diamond particles of the Ni–diamond coating was relatively uniform, and the content was relatively high. The internal stress of the coating prepared by the composite electrodeposition technology was very low. With the incorporation of the diamond particles, the surface roughness of the coating tended to decrease. The wear experiment showed that the wear scar diameter of the corresponding glass ball for the Ni coating was 1.775 mm and the roughness was 13.88 ± 2.811 µm, while that for the Ni–diamond coating was 2.680 mm and 8.35 ± 0.743 µm, respectively, indicating that the tool coating with uniform diamond particles had a strong ability to process workpieces with significantly improved surface quality. The particle press-in mechanism not only improved the wear resistance of the coating, but helped to prolong the service life of the tool. The results of the EIS test and Tafel curves showed that the Ni–diamond coating had a lower corrosion current, and the corrosion resistance of the coating surface was improved. The experimental results showed that the micro-diamond coating prepared by the composite electrodeposition technology had good bonding strength, low internal stress, and significantly improved wear resistance and corrosion resistance.

Background Topoisomerases are nuclear enzymes that get to the bottom of topological troubles related with DNA all through a range of genetic procedures. More and more studies have shown that topoisomerase-mediated DNA cleavage plays crucial roles in tumor cell death and carcinogenesis. There is however still a lack of comprehensive multi-omics studies related to topoisomerase family genes from a pan-cancer perspective. Methods In this study, a multiomics pan-cancer analysis of topoisomerase family genes was conducted by integrating over 10,000 multi-dimensional cancer genomic data across 33 cancer types from The Cancer Genome Atlas (TCGA), 481 small molecule drug response data from cancer therapeutics response portal (CTRP) as well as normal tissue data from Genotype-Tissue Expression (GTEx). Finally, overall activity-level analyses of topoisomerase in pan-cancers were performed by gene set variation analysis (GSVA), together with differential expression, clinical relevancy, immune cell infiltration and regulation of cancer-related pathways. Results Dysregulated gene expression of topoisomerase family were related to genomic changes and abnormal epigenetic modifications. The expression levels of topoisomerase family genes could significantly impact cancer progression, intratumoral heterogeneity, alterations in the immunological condition and regulation of the cancer marker-related pathways, which in turn caused the differences in potential drugs sensitivity and the distinct prognosis of patients. Conclusion It was anticipated that topoisomerase family genes would become novel prognostic biomarkers for cancer patients and provide new insights for the diagnosis and treatment of tumors.