Physical education is an important part of a university, and the satisfaction of college students for physical education directly determines the teaching effect of physical education. Therefore, it is of great significance to understand college students’ satisfaction with physical education and its influencing factors for improving the level of physical education. In this paper, by means of multistage sampling, probability sampling according to scale and random equidistant sampling, 7 main campuses, including 36 subcampuses, are selected for data entry, cleaning, and calculation by using the college physical education teaching system. Through the investigation of 1752 students, the results show that there are significant differences in grade, gender, cognition, credit, sense of responsibility, and teaching content ( P > 0.05 ), which are all factors affecting college students’ satisfaction. Cognition, grades, credits, and make-up test rate are the main influencing factors, with the influence degree ranging from 1 to 3, and there are significant differences in OR value and P value. Therefore, in the process of physical education, we should pay attention to the above-mentioned influencing factors, effectively reduce the occurrence rate of make-up examination and reexamination, adjust unreasonable teaching content, and improve students’ satisfaction with physical education.