Background：The Gelsemium elegans alkaloids have been used in animal feed additives and already achieved terrific results in the pig’s gut health and weight gain. A series of studies have been conducted to explore the mechanisms by which it improves gut health and promotes growth. However, hardly any research has been done in the metabolic changes G. elegans alkaloids elicit in pork muscle. Angiopoietin-like 4 (ANGPTL4), an inhibitor of lipoprotein lipase-mediated plasma triglyceride clearance, is transcribed at a higher rate relative to others in G. elegans alkaloids non-feeding muscle than in G. elegans alkaloids feeding muscle.Methods：A total of 24 muscle tissues and corresponding blood samples were collected from pigs and Target RNA was extracted using TRIzol reagent according to the manufacturer's instructions. Histological and immunofluorescence analysis combined with Western blot analysis were utilized to further explore the possible mechanism of ANGPTL4 and peroxisome proliferator-activated receptor-δ(PPARD).Results： This phenomenon results from that ANGPTL4 in G. elegans alkaloids non-feeding muscle is mediated by elevated plasma free fatty acids via PPARD, presumably leading to reduced local uptake of plasma triglyceride-derived fatty acids and their sparing for use by feeding with G. elegans alkaloids. In contrast, the transcription of ANGPTL4 in G. elegans alkaloids feeding muscle likely is counteracted via lipoprotein lipase(LPL)-mediated negative-regulation, promoting the use of plasma triglycerides as fuel for active muscles. Conclusion：The experimental result indicates that the muscle and the local regulation of ANGPTL4 via PPARD and LPL have critical roles in governing lipid homeostasis during the feeding process of pigs and will shed light on the molecular mechanism discovered by G. elegans alkaloids.