Objective: Cardiac paragangliomas are rare neuroendocrine tumors. Early surgical treatment improves clinical symptoms and prolongs survival. We review our experience in 17 patients who underwent surgical resection for functional cardiac paraganglioma.Methods: Seventeen patients underwent surgery for functional cardiac paraganglioma from 2004 to 2017 were identified. Clinical data and long-term outcomes were extracted and analyzed.Results: All 17 patients with cardiac paraganglioma (11 males) with a median age of 35 years (range, 11 to 51 years) were hormonally functional and underwent operations. A 24-hour urine catecholamine assay documented elevation of norepinephrine, epinephrine, and dopamine. Tumors were determined with octreotide scintiscan in all 17 patients and metaiodoben-zylguanidine scintigraphy in 10 of 15 patients. Tumor sites were right atrioventricular groove in 4 patients, between the main arteries in 10 patients, and interatrial groove in 4 patients. All patients underwent complete resection. Concomitant surgeries were: structural reconstruction in 16 patients (94%) and coronary artery bypass graft in 8 patients (47%). One patient had a history of incomplete resection of an intra-atrial tumor. One patient died postoperatively. Operative mortality is 5.8%. During follow-up (mean, 6.5 years; range, 1.5 to 14.2 years), 16 patients are alive in functional class I or II. Two patients developed tumor recurrence and 14 remained symptom-free with normal urinary catecholamines.Conclusions: With a multidisciplinary treatment, early diagnosis, complete resection for the tumor along with complex reconstruction is achievable for most patients, and it offers promising long-term survival.

Relative to the pharmacopoeia of high performance liquid chromatography (HPLC), establishing detection of puerarin in radix puerariae micellar electro kinetic chromatography (MEKC). Studying on buffer pH, borax concentration, separation voltage, SDS concentration of chromatographic conditions on the puerarin MEKC separation effect of influence, determined the best capillary electrophoresis conditions: test 250 nm wavelength, buffer of pH 9.0 0.1M KH2PO4solution-20 mM borax solution, separation voltage 18 kV, 40 mmol/L SDS concentration, 4.5 min retention time., grained puerarin MEKC standard equation: Y=36.522X-471.9, R2=0.9994(N=5), the puerarin concentration in 550 μg/mL to 750 μg/mL range comes out a good relationship. Get the lowest detection limit with 13.74 μg/mL. Puerarin content of the total isoflavone detected by MEKC was 45.35% that similar to 42.25% detected by HPLC. The method was stable, accurate, sensitive, possessed organic solvent consumption. The research results have applications in puerarin and its product quality evaluation system and promote isoflavonoids detection.

Combine thin layer chromatography ( TLC ) with high performance liquid chromatography ( HPLC ) detection of kudzu root isoflavones, to establish a rapid and accurate method to determine the component content of Puerarin and daidzin in Radix Puerariae.Though study on the optimization analysis of Puerarin Content in Radix Puerariae and daidzin content chromatography in a variety of conditions, including TLC developing solvent and options of coloration, and HPLC chromatographic separation conditions and detection characteristics of TLC and HPL, to get the best Chromatographic separation conditions and make a comparison with Puerarin pharmacopoeia test methods.The experimental results show that: TLC has the advantages of simple operation, rapid, reproducible, rapid identification of Puerarin and daidzin , HPLC with good stability, great accuracy and high precision that can be detected in puerarin and daidzin content. TLC and HPLC is a simple, fast, accurate method and contribute to the establishment of puerarin medicine standardized quality evaluation system and comprehensive utilization and development of Pueraria resources.