Previous studies have demonstrated the effects of sclerostin antibody (Scl-Ab) and parathyroid hormone (1-34, PTH) on healing in osteoporosis; however, reports about the combined effects of Scl-Ab plus PTH on osteoporosis are limited. This study was designed to investigate the impact of combined treatment with Scl-Ab and PTH on osteoporosis healing in ovariectomized (OVX) rats. After bilateral ovariectomy, 12 weeks were allowed to pass for the establishment of standard conditions for osteoporosis in animal models. The rats then randomly received a vehicle (control), Scl-Ab (25 mg/kg body weight, twice weekly), PTH (60 μg/kg, three times per week) or PTH plus Scl-Ab until death at 12 weeks. The blood and distal femurs of the rats were harvested for evaluation. The results of treatment for osteoporosis were evaluated by serum analysis, histology, microcomputed tomography (micro-CT) and biomechanical tests. Results from this study indicated that PTH + Scl-Ab had stronger effects on the prevention and treatment of osteoporosis than either of the monotherapies in OVX rats. The PTH + Scl-Ab produced the strongest effects on bone volume fraction (BV/TV), bone trabecular thickness (Tb.Th), trabecular number (Tb.N) and trabecular spacing (Tb.Sp), bone mineral density (BMD) and strength of distal femurs and increased the levels of procollagen type I N‑terminal propeptide (PINP) and osteocalcin. In contrast, monotherapy with PTH or Scl-Ab showed no differences between treated groups in the assessment of the metaphysis of contralateral femurs by histology, serum, biomechanical tests and micro-CT. These results seem to indicate that Scl-Ab plus PTH has an additive effect on osteoporosis in OVX rats.

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By using a new N-heterocyclic building block, 5,5′-di(pyridin-2-yl)-3,3′-bi(1,2,4-triazole) (2,2′-H 2 dbpt), five novel coordination complexes, Fe 4 (2,2′-Hdbpt) 2 Cl 6 (H 2 O) 6 (1), [Fe(2,2′-H 2 dbpt)Cl 2 ·H 2 O] n (2), [Fe(2,2′-H 2 dbpt)Cl 2 ] n (3), [Fe 2 (2,2′-H 2 dbpt)Cl 4 ] n (4), and [Fe(2,2′-H 2 dbpt) 0.5 Cl 2 ] n (5), based on a Fe(II)/Cl system with diversiform connectivity from zero-to two-dimensional (2D) were constructed successfully. By regulating the metal−ligand ratio and solvents, 2,2′-H 2 dbpt changed various coordination modes. Consequently, 1 reveals a discrete Fe 4 (2,2′-Hdbpt) 2 Cl 6 (H 2 O) 6 supramolecule. 2 and 3 are pseudo-polymorphic. 2 reveals a helical chain based on the cis-FeCl 2 N 4 unit and the cis-bridging 2,2′-H 2 dbpt ligand, while 3 reveals a straight-chain based on the trans-FeCl 2 N 4 unit and the transbridging 2,2′-H 2 dbpt ligand. 4 and 5 are also supramolecular isomers. Different from 2 and 3, both of them have a cis-FeCl 2 N 4 unit. Owing to the different rotation angles of pyridine rings in 2,2′-H 2 dbpt, 4 and 5 reveal a one-dimensional modified trapezoid chain based on the trans-bridging 2,2′-H 2 dbpt ligand and a 2D wave-like layer based on the cis-bridging 2,2′-H 2 dbpt ligand, respectively. Furthermore, compound 4 could also be viewed as a wave banded chain constructed by 3 with μ 2 -Cl ions replaced by the terminal Cl ions and water molecules from another perspective. 1 reveals weak antiferromagnetic behavior. 2, and 3 both reveal paramagnetic behavior, while 4 and 5 both reveal ferromagnetic behavior. These results indicate that 2,2′-H 2 dbpt is an excellent multi-connection linker to construct supramolecular coordination complexes with interesting structures and properties. ■ INTRODUCTIONThe design and synthesis of supramolecular coordination complexes have attracted remarkable attention in the realm of supramolecular chemistry and crystal engineering, not only owing to their appealing structural and topological novelty but also because of their tremendous potential applications in gas storage and separation, 1−5 electrical conduction, 6−9 luminescence materials, 10−20 molecular magnets, 21−26 and heterogeneous catalysis. 27−32 However, how to rationally design and synthesize supramolecular coordination complexes with the desired structure and properties is still a challenge up to now. The assembly of such supramolecular systems may be easily affected by many factors, including the coordination geometry of the central metal ions, solvents, 33−35 ligand structure, metal− ligand ratio, 36,37 counterions, 38−40 pH, 41−44 temperature, 45,46 and so on. Among the reported studies, much effort has been focused on the rational design and controlled synthesis of coordination polymers using multidentate ligands such as polycarboxylate acids, phosphonic acids, and organic ligands containing heterocyclic triazolyl groups. 47 5,5′-Di(pyridin-2-yl)-3,3′-bi(1,2,4-triazole)(2,2′-H 2 dbpt) may be a excellent multidentate ligand to construct supramolecular coordination complexes...