To better understand the ensiling characteristics of sorghum stalk, the dynamic changes of fermentation parameters, nutrient components and bacterial community of sorghum stalk silage were analyzed by intermittently sampling on day 0, 1, 3, 7, 14, 28, and 56 of ensiling duration. The results showed that high-moisture sorghum stalk was well preserved during ensiling fermentation, with the DM loss of 4.10% and the little difference between the nutrients of sorghum stalk before and after ensiling. The pH value of silage declined to its lowest value of 4.32 by Day 7 of ensiling, and other fermentation parameters kept steady since Day 28 of ensiling. The amplicon sequencing analysis revealed that the alpha diversity parameters of silage bacterial community including Shannon index, observed features, Pielou evenness and Faith PD gradually declined (P < 0.01) with ensiling duration. Principal coordinate analysis (PCoA) revealed that bacterial profiles of raw material would experience a succession becoming a quite different community during ensiling fermentation. Taxonomic classification revealed a total of 10 and 173 bacterial taxa at the phylum and genus level, respectively, as being detected with relative abundances higher than 0.01% and in at least half samples. LEfSe analysis revealed that 26 bacterial taxa were affected by sampling timepoint (P < 0.05 and LDA score > 4). When focusing on the dynamic trend of silage bacterial taxa, lactic acid bacteria successfully dominated in the bacterial community on Day 1 of ensiling, and the bacterial community almost came to a plateau by Day 28 of ensiling, with Lactobacillus and Leuconostoc as the dominant genera. In a word, the succession of fermentation parameters, nutrient components and bacterial community indicate a successful dominance establishment of LAB and a fast advent of fermentation plateau, suggesting that high-moisture sorghum stalk can be ensiled directly, but the pH of mature silage is a little high.

This paper presents experimental and numerical investigations on progressive collapse behavior of a two-story glulam frame when the side column is exposed to ISO834 standard fire. The collapse mechanism initiated by fire is identified. The experimental results show that the progressive collapse of a glulam frame could be described for three stages, namely bending effect stage, catenary effect stage and failure stage, respectively. These stages are discussed in detail to understand the structural behavior before and during collapse. It is demonstrated that the entire frame slopes towards the side of the heated column, and the "overturning" collapse occurs eventually. The catenary effect of beams is the main reason for the progressive collapse of the frame. In addition, a finite element model of a glulam frame is established to simulate the progressive collapse behavior. The effects of axial loads on the columns are summarized. The numerical simulation results agree well with the experimental results, which could verify the effectiveness and practicability of finite element simulation. Furthermore, the progressive collapse resistance of the frame in practical design were proposed.