Deceit often occurs in questionnaire surveys, which leads to the misreporting of data and poor reliability. The purpose of this study is to explore whether eye-tracking could contribute to the detection of deception in questionnaire surveys, and whether the eye behaviors that appeared in instructed lying still exist in spontaneous lying. Two studies were conducted to explore eye movement behaviors in instructed and spontaneous lying conditions. The results showed that pupil size and fixation behaviors are both reliable indicators to detect lies in questionnaire surveys. Blink and saccade behaviors do not seem to predict deception. Deception resulted in increased pupil size, fixation count and duration. Meanwhile, respondents focused on different areas of the questionnaire when lying versus telling the truth. Furthermore, in the actual deception situation, the linear support vector machine (SVM) deception classifier achieved an accuracy of 74.09%. In sum, this study indicates the eye-tracking signatures of lying are not restricted to instructed deception, demonstrates the potential of using eye-tracking to detect deception in questionnaire surveys, and contributes to the questionnaire surveys of sensitive issues.
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