Rice blast caused by Magnaporthe oryzae ( M. oryzae ) is a major threat to global rice production. In recent years, small interference RNAs (siRNAs) and host-induced gene silencing (HIGS) has been shown to be new strategies for the development of transgenic plants to control fungal diseases and proved a useful tool to study gene function in pathogens. We here tested whether in vitro feeding artificial siRNAs (asiRNAs) could compromise M. oryza e virulence and in vivo HIGS technique could improve rice blast resistance. Our data revealed that silencing of M. oryzae MoAP1 by feeding asiRNAs targeting MoAP1 (i.e., asiR1245, asiR1362, and asiR1115) resulted in inhibited fungal growth, abnormal spores, and decreased pathogenicity. Among the asiRNAs, asiR1115 was the most inhibitory toward the rice blast fungus. Conversely, the asiRNAs targeting three other genes (i.e., MoSSADH , MoACT , and MoSOM1 ) had no effect on fungal growth. Transgenic rice plants expressing RNA hairpins targeting MoAP1 exhibited improved resistance to 11 tested M. oryzae strains. Confocal microscopy also revealed profoundly restricted appressoria and mycelia in rice blast-infected transgenic rice plants. Our results demonstrate that in vitro asiRNA and in vivo HIGS were useful protection approaches that may be valuable to enhance rice blast resistance.

The Experimental Advanced Superconducting Tokamak (EAST) has recently achieved a variety of H-mode regimes with different edge-localized mode (ELM) dynamics, including type-I ELMs, compound ELMs, which are manifested by the onset of a large spike followed by a sequence of small spikes on Dα emissions, usual type-III ELMs, and very small ELMs. This newly observed very small ELMy H-mode appears to be similar to the type-II ELMy H-mode, with higher repetition frequency (∼1 kHz) and lower amplitude than the type-III ELMy H-mode, exhibiting an intermediate confinement level between type-I and type-III ELMy H-modes. The energy loss and divertor power load are systematically characterized for these different ELMy H-modes to provide a physics basis for the next-step high-power long-pulse operations in EAST. Both type-I and compound ELMs exhibit good confinement (H98(y,2) ∼ 1). A significant loss of the plasma stored energy occurs at the onset of type-I ELMs (∼8%) and compound ELMs (∼5%), while no noticeable change in the plasma stored energy is observed for the small ELMs, including both type-III ELMs and very small ELMs. The peak heat flux on divertor targets for type-I ELMs currently achieved in EAST is about 10 MW m−2, as determined from the divertor-embedded triple Langmuir probe system with high time resolution. As expected, type-III ELMs lead to much smaller divertor power loads with a peak heat flux of about 2 MW m−2. Peak power loads for compound ELMs are between those for type-I and type-III ELMs. It is remarkable that the new very small ELMy H-modes exhibit even lower target power deposition than type-III ELMs, with the peak heat flux generally below 1 MW m−2. These very small ELMs are usually accompanied by broadband fluctuations with frequencies ranging from 20 to 50 kHz, which may promote particle and power exhaust throughout the very small ELMy H-mode regime.

BackgroundA number of field studies have demonstrated that the yield potential of hybrid rice cultivars is higher than that of inbred cultivars, although the magnitude of difference between hybrid and inbred cultivars at different yield levels has not been described. The objective of this study is to compare the yield increase potential at different yield levels between hybrid and conventional rice. Ten field experiments were conducted at five locations in southern China in 2012 and 2013. At each location, two hybrid and two inbred cultivars were grown at three N levels: high (225 kg/hm2), moderate (161–191 kg/hm2) and the control, zero N (0 kg/hm2).ResultsHybrid rice yielded approximately 8 % more grain than did inbred cultivars in Huaiji, Binyang and Haikou; approximately 7 % more in Changsha; and approximately 19 % more in Xingyi. The high grain yields observed for hybrid rice cultivars were attributed to high grain weight and biomass accumulation at maturity. On average, rice yields were approximately 6.0–7.5 t ha−1 (medium yield) in Huaiji, Binyang and Haikou; approximately 9.0 t ha−1 in Changsha (high yield); and approximately 12.0 t ha−1 (super high yield) in Xingyi. The yield gaps among Huaiji, Binyang and Haikou and Changsha were attributed to the differences in spikelets m−2 and biomass production, whereas the yield gap between Changsha and Xingyi was caused by the differences in grain-filling percentage, grain weight and harvest index. The differences in biomass production among sites were primarily due to variation in crop growth rate induced by varied temperatures and accumulative solar radiation.ConclusionsThe yield superiority of hybrid rice was relatively small in comparison with that of inbred cultivars at medium and high yield levels, but the difference was large at super high yield levels. Improving rice yields from medium to high should focus on spikelets m−2 and biomass, whereas further improvement to super high level should emphasize on grain-filling percentage, grain weight and harvest index. Favorable environmental conditions are essential for high yields in hybrid rice.

A new turbulence-flow cycle state has been discovered after the formation of a transport barrier in the H-mode plasma edge during a quiescent phase on the EAST superconducting tokamak. Zonal-flow modulation of high-frequency-broadband (0.05-1 MHz) turbulence was observed in the steep-gradient region leading to intermittent transport events across the edge transport barrier. Good confinement (H 98y,2 $ 1) has been achieved in this state, even with input heating power near the L-H transition threshold. A novel model based on predator-prey interaction between turbulence and zonal flows reproduced this state well. V