Microbial induced carbonate precipitation (MICP) provides an alternative method to stabilize the soil. To further improve the reinforcement effect, this study aims to propose a strategy by incorporating the mixing MICP method with pretreatment procedure. A series of laboratory tests were performed to investigate the preparation parameters (including the moisture content and dry density of the soil, the concentration of urea and CaCl2 in cementation solution), the engineering properties, the CaCO3 distribution as well as the mineralogical and micro structural characteristics of pretreatment-mixing MICP reinforced soil (PMMRS). Based on the orthogonal experiment results, the optimum preparation parameters for PMMRS were determined. The UCS of PMMRS was more strongly dependent on the moisture content and concentration of CaCl2 than the concentration ratio of CaCl2 to urea. Moreover, it was testified that incorporation of pretreatment procedure improved the stabilization effect of traditional mixing MICP method on the clayed sand (CLS). The UCS of PMMRS specimen was increased by 198% and 78% for the pure CLS and the simple mixing MICP reinforced soil, respectively. Furthermore, the CaCO3 products generated consisted of the aragonite, calcite and vaterite, which distributed unevenly inside the specimen no matter the lateral or vertical direction. The reason for the uneven distribution might be that oxygen content varied with the regions in different directions, and hence affected the mineralization reaction. In addition, the mineralization reaction would affect the pore structure of the soil, which was highly related to the stabilization effect of MICP reinforced soil.