As a nanozyme, gold nanoparticles have some advantages compared with natural enzymes, such as stable structure, adjustable catalytic activity, multifunctionality, and recyclability. Due to their special dimension, they are easy to aggregate rapidly and lose their catalytic performance when exposed to normal saline or special pH environment. To avoid such a situation, Au@PNIPAm nanozymes with core−shell structure are constructed and their mimic peroxidase and glucose oxidase enzymatic activities are investigated. Kinetic examinations manifest that Au@PNIPAm nanozymes exhibited a high affinity for 3,3,5,5tetramethylbenzidine (TMB), hydrogen peroxide (H 2 O 2 ), and glucose. These predominant peroxidase-like and glucose-like oxidase Au@PNIPAm catalytic activities are successfully used in the detection of H 2 O 2 or glucose (LOD is 2.43 mM or 5.07 mM). Otherwise, the potential Au@PNIPAm nanozymes are provided with a clear ability for decomposing the intracellular H 2 O 2 in living cells. And it could protect cells from oxidative stress damage with inducing by H 2 O 2 . Therefore, it is easy to consider that Au@PNIPAm nanozymes show a certain possibility to retard cell senescence and increase the production of the hydroxyl radical which could prevent carcinogenesis of the cell.

Thermo-responsive nanogels, Poly (NIPAM-AAM) were prepared by a facile method of free radical one-pot precipitation method based on monomeric N-isopropylacrylamide (NIPAM). At the same time, the surface carboxyl group-modified fluorescent...