Magnesium (Mg) plays important roles in photosynthesis, sucrose partitioning, and biomass allocation in plants. However, the specific mechanisms of tea plant response to Mg deficiency remain unclear. In this study, we investigated the effects of Mg deficiency on the quality constituents of tea leaves. Our results showed that the short-term (7 days) Mg deficiency partially elevated the concentrations of polyphenols, free amino acids, and caffeine but decreased the contents of chlorophyll and Mg. However, long-term (30 days) Mg-deficient tea displayed decreased contents of these constituents. Particularly, Mg deficiency increased the index of catechins' bitter taste and the ratio of total polyphenols to total free amino acids. Moreover, the transcription of key genes involved in the biosynthesis of flavonoid, caffeine, and theanine was differentially affected by Mg deficiency. Additionally, short-term Mg deficiency induced global transcriptome change in tea leaves, in which a total of 2522 differentially expressed genes were identified involved in secondary metabolism, amino acid metabolism, and chlorophyll metabolism. These results may help to elucidate why short-term Mg deficiency partially improves the quality constituents of tea, while long-term Mgdeficient tea may taste more bitter, more astringent, and less umami.

The low-permeability conglomerate reservoir in the Mahu Sag has great resource potential, but its strong heterogeneity and complex microscopic pore structure lead to a high oil-gas decline ratio and low recovery ratio. Clarifying the migration rule of crude oil in microscopic pore throat of different scales is the premise of efficient reservoir development. The low-permeability conglomerate reservoir of the Baikouquan Formation in the Mahu Sag is selected as the research object, and two NMR experimental methods of centrifugal displacement and imbibition replacement are designed to reveal the differences in the migration rule of crude oil in different pore throats. According to the lithology and physical properties, the reservoirs in the study area can be divided into four categories: sandy grain-supported conglomerates, gravelly coarse sandstones, sandy-gravelly matrix-supported conglomerates and argillaceous-supported conglomerates. From type I to type IV, the shale content of the reservoir increases, and the physical property parameters worsen. Centrifugal displacement mainly produces crude oil in large pore throats, while imbibition replacement mainly produces crude oil in small pores. In the process of centrifugal displacement, for type I reservoirs, the crude oil in the pore throats with radii greater than 0.5 μm is mainly displaced, and for the other three types, it is greater than 0.1 μm. The crude oil in the pore throats with radii of 0.02–0.1 μm, which is the main storage space for the remaining oil, is difficult to effectively displace. The crude oil in the pore throats with radii less than 0.02 μm cannot be displaced. The two experimental methods of centrifugation and imbibition correspond to the two development methods of displacement and soaking in field development, respectively. The combination of displacement and soaking can effectively use crude oil in the full-scale pore throat space to greatly improve the recovery of low-permeability conglomerate reservoirs.