Five coordination sites of Cu(ii) cluster were found in VNU-18 that showed highly efficient catalytic property for the oxidative C–H activation via N–H bonds.

Ligands interacting with abasic (AP) sites in DNA may generate roadblocks in base‐excision DNA repair (BER) due to indirect inhibition of DNA repair enzymes (e.g., APE1) and/or formation of toxic byproducts, resulting from ligand‐induced strand cleavage or covalent cross‐links. Herein, a series of 12 putative AP‐site ligands, sharing the common naphthalenophane scaffold, but endowed with a variety of substituents, have been prepared and systematically studied. The results demonstrate that most naphthalenophanes bind to AP sites in DNA and inhibit the APE1‐induced hydrolysis of the latter in vitro. Remarkably, their APE1 inhibitory activity, as characterized by IC50 and KI values, can be directly related to their affinity and selectivity to AP sites, as assessed by means of fluorescence melting experiments. On the other hand, the molecular design of naphthalenophanes has a crucial influence on their intrinsic AP‐site cleavage activity (i.e., ligand‐catalyzed β‐ and β,δ‐elimination reactions at the AP site), as illustrated by the compounds either having an exceptionally high AP‐site cleavage activity (e.g., 2,7‐BisNP‐S, 125‐fold more efficacious than spermine) or being totally devoid of this activity (four compounds). Finally, the unprecedented formation of a stable covalent DNA adduct upon reaction of one ligand (2,7‐BisNP‐NH) with its own product of the AP‐site cleavage is revealed.