No abstract

Colonization of plant roots by root knot and cyst nematodes requires a functional ethylene response pathway. However, ethylene plays many roles in root development and whether its role in nematode colonization is direct or indirect, for example lateral root initiation or root hair growth, is not known. The temporal requirement for ethylene and localized synthesis of ethylene during the life span of soybean cyst nematode (SCN) on soybean roots was further investigated. Although a significant increase in ethylene evolution was not detected from SCN-colonized roots, the concentration of the immediate precursor to ethylene, 1-aminocyclopropane-1-carboxylic acid (ACC), was higher in SCN-colonized root pieces and root tips than in other parts of the root. Moreover, expression analysis of 17 ACC synthase (ACS) genes indicated that a select set of ACS genes is expressed in SCN-colonized root pieces that is clearly different from the set of genes expressed in non-colonized roots or root tips. Semi-quantitative real-time PCR indicated that ACS transcript accumulation correlates with the high concentration of ACC in root tips. In addition, an ACS-like sequence was found in the public SCN nucleotide database. Acquisition of a full-length sequence for this mRNA (accession GQ389647) and alignment with transcripts for other well-characterized ACS proteins indicated that the nematode sequence is missing a key element required for ACS activity and therefore probably is not a functional ACS. Moreover, no significant amount of ACC was found in any growth stage of SCN that was tested.

No abstract

Ethylene controls many beneficial responses in plants but also promotes chlorosis, senescence, disease, and fruit over‐ripening. The present study compared previously isolated ethylene‐insensitive lines of soybean [Glycine max (L.) Merr.] to their isogenic, ethylene‐sensitive parent with respect to disease resistance, seed yield, and other field performance traits. In laboratory tests, ethylene insensitivity reduced root colonization by soybean cyst nematode. Using healthy young plants, ethylene‐insensitivity also reduced ethylene‐activated leaf chlorosis and abscission. However, in the field, leaf chlorophyll and late‐season senescence were not altered, suggesting that ethylene is not a main determinant of late‐season senescence of soybean leaves. Field studies also revealed no changes in susceptibility to Septoria brown spot disease (caused by Septoria glycines Hemmi), flowering date, plant height, or seed total protein and oil concentration. Field studies did demonstrate elevated susceptibility to white mold disease [Sclerotinia sclerotiorum (Lib.) deBary], poor stand establishment in some but not all environments, altered plant architecture, and earlier maturity date in the ethylene‐insensitive lines. Seed yield was notably undependable, being similar to the parental line in some field locations but significantly reduced in most environments. To avoid these negative impacts on overall performance, manipulation of plant ethylene responses should be targeted to specific tissues, growth stages, or growth environments.

This research explored the effects of biochar on slope runoff and sediment transport processes and the hydrodynamic mechanism of rill erosion under the seasonal freeze–thaw climate in the black soil area of Northeast China. The four slopes of 1.8, 3.6, 5.4 and 7.2° were set, corn straw biochar was used, and three biochar contents of 0 kg m−2 (B0 treatment), 6 kg m−2 (B6 treatment) and 12 kg m−2 (B12 treatment) were applied. The experimental plot was placed outdoors to simulate the freeze–thaw cycle of sloping farmland under natural conditions. Three artificial simulated rainfall tests were carried out before the end of seasonal freeze–thaw cycles and spring sowing date (May) in 2018 and 2019. The sediment transport process of runoff and the variation of hydrodynamic parameters in rills were analyzed under one and two seasons of freezing and thawing in natural outdoor conditions. The results show that biochar has a positive effect on reducing rainfall runoff and soil loss after one year and two years of seasonal freezing and thawing. The effect of biochar on the sediment concentration of slope runoff increased with increasing application time; in the second year, the B6 and B12 treatments reduced the sediment concentration by 5.5–14.8% and 3.3–13.6%, respectively, compared with the values of the first year. The Reynolds number (Re) in the rill flow after the B6 and B12 treatments decreased with increasing duration, which effectively reduced the turbulence degree of the flow on the rill of the slope. With the increase in duration, the rill critical erosion power increased; in 2018 and 2019, the critical shear force, critical runoff power and critical unit runoff power were 0.403 Pa, 0.098 m s−1, and 0.002 N m−1 and 0.497 Pa, 0.124 m s−1, and 0.003 N m−1, respectively. This result indicates that increasing the duration and number of seasonal freeze–thaws can promote the development of biochar control of the runoff and sediment processes on slope and rill development.