Abstract. Iron (III) tetrasulfophthalocyanine (Fe (III) PcTs) has been used as a biomimetic enzyme to catalyze the oxidation of 2-CP. Fe (III) PcTs has been Prepared and characterized, including FT-IR, SEM, XRD, UV-Vis and element analysis of Fe (III) PcTs. It showed Fe (III) PcTs has high purity. We studied the oxidation of 2-CP catalyzed by Fe (III) PcTs, the intensities of absorption at 510 nm.

An optic determination method based on the oxidation of 2-chlorophenol (2-CP) catalyzed by Iron (III) Tetrasulfophthalocyanine (Fe (III) PcTs) was developed for the determination of 2-CP. The optical oxygen sensing film containing fluorescence indicator Ru (bpy) 3 Cl 2 was used to detect the consumption of oxygen in solution. A lock-in amplifier was used for detecting the lifetime of the oxygen sensing film by measuring the phase delay of the sensor head. Under the optimal conditions (pH6.0, 25 o C, Fe (III) PcTs concentration of 0.62 mg/mL), the linear detection range and response time of the biosensor were 1.0×10 -6 -9.0×10 -6 mol/L and 230s, respectively. The sensor displayed high selectivity and could be used as an effective tool in analyzing 2-CP concentration in practical water samples.

An optical fiber sensor was designed and fabricated for the determination of 2-Chlorophenol (2-CP) based on 2-CP oxidation by oxygen with the catalysis of iron(III) tetrasulfophthalocyanine (Fe(III)PcTs). A lock-in amplifier was used for detecting the lifetime of the oxygen sensing film by measuring the phase delay of the sensor head. The linear detection range of the biosensor was 1.0×10-6 -9.0×10-5 mol/L and the response time was 226s. The sensor displays high selectivity, good repeatability and stability, which shows good application prospect in the analysis of 2-CP concentration in actual water samples.