No abstract

No abstract

Due to efficient performance and stability, nanozymes have recently attracted much attention in bioreaction.In this work, a facile approach for preparing N-doped hollow carbon sheres (N-HCSs) by using CaCO 3 spheres as green template and polydoamine as nitrogen and carbon sources was reported. Morphologies and structures of the samples were characterized. Using TMB (3,3,5,5 tetramethylbenzidine) as a substrate, UV spectra photometry was used to investigate its oxidase-like activity and catalytic mechanism. The results showed that N-HCSs displayed oxidase-like activity. The oxidase-like activity of N-HCSs increased by three times after activation by KOH. These enzymes conform to the Michaelis-Menten kinetic equation, and the K m constant before and after activation were 0.105 and 0.083, respectively, indicating good affinity for substrates. The data demonstrate that it is superoxide anion

No abstract

Due to high stability and sensitivity, nanozymes have recently attracted much attention in bioreaction. In this work, a facile approach for preparing N-doped hollow carbon sheres (N-HCSs) by using CaCO3 spheres as green template and polydoamine as nitrogen and carbon sources was reported. The morphology and structure of the samples were characterized. Using TMB (3,3,5,5' tetramethylbenzidine) as a substrate, UV sectrohotometry was used to investigate its oxidase-like activity and catalytic mechanism. The results showed that N-HCSs displayed oxidase-like activity. The oxidase-like activity of N-HCSs was increased by three times after activation by KOH. These enzymes conform to the Michaelis-Menten kinetic equation, and the Km constant before and after activation were 0.1094 and 0.0825, respectively, indicating good affinity for substrates. The data demonstrated that it is superoxide anion (O2• -) that plays a major role in catalytic reaction. All these data provides a theoretical basis for the design and preparation of high activity oxidase-mimicking enzymes.