This work was carried out in collaboration between both authors. Author YAS designed the study, performed the statistical analysis, wrote the protocol, managed the analyses of the study, literature searches and supervised the laboratory work. Author AAA wrote the first draft of the manuscript, managed literature searches and carried the laboratory work. Both authors read and approved the final manuscript.

Aim of the Study: To assess the bacterial contamination in drinking water sources in Khartoum/ Sudan. Place of Study: Central Veterinary Research Laboratory/ Bacteriology Department. Study Design: One hundred water samples were collected from the three localities of Khartoum state (Khartoum= 33, Omdurman= 34,   Khartoum north [Bahri] =25) and 8 from different companies of water supply. Methodology: Fifty four Samples were collected from surface water and (38) from ground water [well]. These samples transported to bacteriology lab for microbiological analyses using filtration method and new technique Colilert and Pseudalert kits which used for the first time in Sudan. Results: Filtration method revealed different bacterial species, they were: Escherichia coli, Klebsiella pneumoniae, Enterobacter aerogene Enterobacter sakazaki, Enterobacter cloacae, Serratia marinoruba, Proteus mirabilis, Salmonella spp. Raoultella terrigena\planticola, Orchobacter anthrobi, Cronobater spp., Aeromonas salmonicida, Aeromonas hydrophilia, Pantoea agglomerans, Vibrio parahaemolyticus. Coliform bacteria, Escherichia coli and Pseudomonas app were detected and most probable numbers (MPN) were counted using the previous kits according to manufacture instructions. Conclusion: The water must be tested before using and quality control technique must be achievable to ensure continuously supply of pure drinking water.

In this study, Staphylococcus aureus was isolated from a draught horse presented to Kassala Veterinary Research Laboratory (KVRL) for investigation and diagnosis. The affected animal was previously treated with Gentamycin and Tetracycline before submitted to this laboratory, but the animal was not cure. Thereafter, the animal was attended to the laboratory; it was suffering from inguinal abscess and some clinical signs. Clinical and laboratory examinations were carried out however, the animal was suddenly fallen and died immediately. Then post-mortem, bacteriological and histopathological examinations were done. S. aureus was isolated as pure culture from the lungs and peritoneum of the necropsied animal. This isolate showed highly sensitivity to Erythromycin and Clindamycin and it was resistant to Penicillin, Cloxacillin, Cefotaxime, Co-Trimoxazole, Cephalexin and Tetracycline. It was concluded from this study, the shock resulted from S. aureus was the causative agent of the death in this case.

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