Poster abstractsthe current PP13 ELISA test and their existence can explain low levels of serum PP13 in women who subsequently develop preeclampsia. In this study we investigated the presence of circulating PP13 mRNA in pregnancies with physiological course of gestation. Methods: For the testing, we developed three various real-time RT-PCR systems (exon 1-2, exon 4, exon 4-UTR) for the detection of normal PP13 mRNA and for its shorter isoforms. The presence of PP13 mRNA was analysed in plasma samples of 16 women during the first and the third trimesters of gestation. Results: The systems were optimised to detect reliably 500, 50 and even 5 copies of placental-derived PP13 in a mixture with redundant PP13 negative RNA plasma sample derived from non-pregnant healthy individuals. However, the detection rate of PP13 mRNA in maternal plasma samples was substantially inconsistent (at the most one or two positive replicates out of 6 tested ones). Conclusions:The early detection of PP13 mRNA in maternal plasma does not represent a suitable method to predict later development of preeclampsia.