Anion exchanger-1 (AE1), an erythroid-specific membrane protein, mediates the Cl À /HCO À 3 exchange across the plasma membrane and regulates intracellular pH. We have found that AE1 was unexpectedly expressed in gastric cancer cells and participated in the tumorigenesis of the cancer. Here, we focus on the induction of AE1 expression and its role in gastric carcinogenesis as well as in the differentiation of K562 cells. The results show that expression of AE1 is not related to genetic mutation or the mRNA level, but rather, that it is modulated by miR-24. miR-24 decreases the expression of AE1 through binding to the 3 0 UTR of AE1 mRNA. Transfection of an miR-24 into gastric cancer cells reduced the elevation of the AE1 protein, which resulted in return of AE1-sequestrated p16 to the nucleus, thereby inhibiting proliferation of the cells. Furthermore, the miR-24 inhibitor cooperated with hemin to induce the expression of AE1 in K562 cells and differentiation of the cells, which is consistent with results obtained from the cells cultured at pH 7.6 or from forced stable expression of AE1. These findings establish a novel regulation of miR-24-related AE1 expression in gastric carcinogenesis and erythropoiesis.
The aim of this article is to provide some basis for the design and assembly of a bolted rotor with curvic couplings. It is well known that the key difference between a bolted rotor with curvic couplings and an integrated one is the contact interface. According to the characteristics of curvic couplings and spindle bolts, the model of a bolted rotor with curvic couplings of the turbine end of a heavy duty gas turbine was built. A method of accurately applying the preload force has been studied in this article. The three-dimensional finite-element contact method was used, non-linear behaviours such as friction and contact were also taken into account, and the dynamic contact between the spindle bolts and the sidewall of turbine wheels was included. The tendency of stress, which involved the rotor, curvic couplings, and the spindle bolts, was determined and the radial slippage trend of curvic teeth was also determined, by investigating the stress distribution and contact behaviour of the bolted rotor with curvic couplings during the course of preload, warm-up, speed-up, and running. It can be seen from the results that the contact stress of curvic couplings is dominant during the course of preload, and the bent stress is dominant when the rotating speed increased to 3000 r/min; the stress inequality on two sides of a tooth is caused by torque, so the stress proportion induced by torque should be restricted to an appropriate level to avoid anisotropy of the rotor.
In this paper, a mathematical model for the developed electromagnetic suspension actuator is established based on measured parameters and test data. A main/inner-loop control strategy is proposed for its active control and the inner loop undertakes the control of a three-phase motor by applying a hysteresis current control method to track the calculated signal by the main loop. With the simplification of the inner-loop control system, the achievable range of control current and energy flow states of the actuator are analysed. Then, owing to the non-linear damping characteristic of the actuator, a hybrid system model which involves real and integer variables is built up for the main loop and optimal control to improve vehicle ride comfort is carried out based on a model predictive control method. Simulations are carried out with bump impulse and random uneven road inputs and the results demonstrate that the electromagnetic suspension with the designed hybrid controller can improve vehicle ride comfort compared with a corresponding passive suspension while energy is consumed from the battery.
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