Introgressive hybridization of common wheat has been and remains an efficient way for enriching its gene pool with resistance genes against environmental stress factors. In this aspect, T genome of the diploid species Amblyopyrum muticum (Boiss.) Eig. is believed to be prospective. The genome substitution amphidip loid Aurotica (AABBTT) with the A and B subgenomes from the common wheat cultivar Aurora and with the T subgenome from the indicated species is characterized by a high level of winter resistance. This trait is asso ciated in wheat with the chromosomes of the fifth homoeologous group. Using a comparative microsatellite analysis of Aurora and Aurotica DNAs, we identified nine SSR loci specific for the wheat D genome, whose primers produced different amplicons from Aurotica DNA. The latter were used in screening F 5 plants (Aurora × Aurotica) for the search of the specimens that carried the 5T chromosome or its fragments in their genomes. Among 234 plants, we detected 24 plants with disomic 5D/5T substitutions, which were the prog eny of five F 4 plants grown from two F 2 plants. A significant number of plants contained the recombinant 5D/5T chromosome. The recombination event could occur in any generation starting with F 2 .