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A comparative study between EHVF (eel herpesvirus in Formosa) and HVA (Herpesairus anguillw) was performed. Similar syncytia were produced by each virus in cell lines infected with the viruses and similar viral yields were obtained. Significant cross-reactivity was observed in neutralization tests between HVA and EHVF but HVA and EFVF were distinctly different from channel catfish virus (CCV). An electrophoretic analysis of the structural proteins of EHVF and HVA produced similar electrophoretic patterns. Western blotting analysis of the viral proteins, using antiserum against EHVF, supported the results obtained in the cross-neutralization test. In conclusion, the isolates of HVA and EHVF are tightly clustered.

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