Tumstatin (Tum) is a powerful angiostatin that inhibits proliferation and induces apoptosis of tumorous vascular endothelial cells. A nonpathogenic and anaerobic bacterium, Bifidobacterium longum (BL), selectively localizes to and proliferates in the hypoxia location within solid tumor. The aims of this study were to develop a novel delivery system for Tum using engineered Bifidobacterium and to investigate the inhibitory effect of Tum on tumor in mice. A vector that enabled the expression of Tum under the control of the pBBADs promoter of BL was constructed and transformed into BL NCC2705 by electroporation. The mouse colon carcinoma cells CT26 (1 × 10(7)/mL) were subcutaneously inserted in the left armpit of BALB/c mice. The tumor-bearing mice were treated with Tum-transformed BL, and green fluorescent protein (GFP)-transformed BL was used as a negative control. The microvessel density (MVD) in the transplanted tumor was determined, and terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end labeling was used to detect apoptosis of vascular endothelial cells in transplanted tumor. The in vitro expression of Tum was examined in BL after l-arabinose induction. Bifidobacterium longum with pBBAD-Tum (BL-Tum) showed significant antitumor effect in tumor-bearing mice. The weight, volume, growth, and MVD, as well as the percentage of apoptotic vascular endothelial cells of transplanted tumors in the tumor-bearing mice treated with Tum-transformed BL were all significantly lower than those in the GFP negative control group. Intragastric administration, injection in tumor and vena caudalis injection of Tum-transformed BL exerted marked antitumor effects in tumor-bearing mice. This is the first demonstration of the utilization of Tum-transformed BL as a specific gene delivery system for treating tumor.

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A novel erbium(III)-carboxylate polymeric complex [{Er(H 2 btec) 2/4 (btec) 3/6 (H 2 O)}·2H 2 O] n , simplified as ECPC, (H 4 btec = 1,2,4,5-benzenetetracarboxylic acid) was synthesized under solvothermal conditions (H 2 O/acetic acid). ECPC obtained was characterized by differential thermal analysis/thermogravimetry (DTA/TG), singlecrystal X-ray diffraction, elemental analysis and FT-IR analysis techniques. The result of single-crystal X-ray diffraction analysis shows that the ECPC crystallizes in monoclinic symmetry, and the space group P2(1)/n, a = 10.6933(15) Å, b = 7.1243(10) Å, c = 17.092(2) Å, α = γ = 90 • , β = 97.109(2) • , V = 1292.1(3) Å 3 , Z = 4, R 1 = 0.0286, wR 2 = 0.0686. ECPC demonstrates a 3-D supramolecular framework containing nine-coordinate erbium centers and channels. The uncoordinated water molecules occupy the channels in ECPC. The results of TG/DTA, IR and elemental analysis performed also give positive information of the proposed crystal structure.