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2,3,5,4'-Tetrahydroxystilbene-2-O-β-glucoside treatment enhanced the renewal ability and proliferative potential of hDPSCs via the AMPK/ERK/SIRT1 axis, which may provide a novel autogenic cell-based therapeutic strategy in regenerative dentistry.

Fe3O4 nanoparticles were loaded into poly-l-lactide (PLLA) with concentrations of 2% and 5%, respectively, using an electrospinning method. In vivo animal experiments were then performed to evaluate the potential of the Fe3O4/PLLA nanofibrous material for bone tissue engineering applications. Bony defects with a diameter of 4 mm were prepared in rabbit tibias. Fe3O4/PLLA nanofibers were grafted into the drilled defects and histological examination and computed tomography (CT) image detection were performed after an eight-week healing period. The histological results showed that the artificial bony defects grafted with Fe3O4/PLLA nanofibers exhibited a visibly higher bone healing activity than those grafted with neat PLLA. In addition, the quantitative results from CT images revealed that the bony defects grafted with 2% and 5% Fe3O4/PLLA nanofibers, respectively, showed 1.9- and 2.3-fold increases in bone volume compared to the control blank sample. Overall, the results suggest that the Fe3O4/PLLA nanofibers fabricated in this study may serve as a useful biomaterial for future bone tissue engineering applications.

Abstract:The aim of this study was to develop an electrospun poly-l-lactide (PLLA) nanofibrous membrane incorporating oleic acid-coated Fe 3 O 4 . The Fe 3 O 4 nanoparticles were prepared using the chemical co-precipitation method, and particle diameters were analyzed using transmission electron microscopy. After mixing the oleic acid-coated Fe 3 O 4 nanoparticles and PLLA, a membrane with nanofibers was manufactured using the electrospinning technique. Our results showed that Fe 3 O 4 nanoparticle diameters fabricated in this study were concentrated at 2-8 nm (84.2%). After magnetizing, there exists an approximately linear relationship between magnetic flux density and membrane thickness (R 2 = 0.7, p < 0.05). NIH-3T3 fibroblast cells cultured on the magnetized Fe 3 O 4 / PLLA nanomembranes exhibited a more spreading and attached phenotype. These results can serve as a reference for future advanced studies.