The main issue today is the provision of safe and high-quality livestock products, taking into account all aspects of food production, from farm to table. In order to preserve the young animals, increase the resistance of the immune system of the organism, prevent the influence of infectious pathogens on macroorganism and the environment, studies have been conducted to determine the microflora circulating in the conditions of the economy. In particular, from the point of view of food safety, E. coli is a dangerous factor affecting their quality, causing various diseases in humans and animals. In the body of a healthy bovine animal, Escherichia coli may be present in an admissible concentration of up to 107 CFUs, Escherichia coli haemolitica is normally absent in the animal body. Bacteriological studies included seeding of the test material on the nutrient medium, their identification and the study of antibiotic susceptibility. For the analysis of the sensitivity of microorganisms to medicinal (antibacterial) substances by disco-diffusion method, they used a nutrient medium of AGV, on which the «lawn» was sown analyzing cultures and used discs with antibacterial preparations. According to the results of bacteriological studies of milk samples, it has been determined that 70% of the examined samples of pathogenic microflora have not been isolated. In 30% of the tested samples, the presence of pathogenic microorganisms such as Staphylococcus aureus and Proteus vulgaris in milk samples was established. Bacteriological studies of vaginal exudate taken from cows after the calving have shown that they have Streptococcus epidermidis, Enterobacter cloacae, Escherišhia coli, Proteus vulgaris and fungus of the genus Aspergillus spp. Investigations of the exudate from the nasal passages and the calving of the calves have revealed that Escherichia coli is present in all the samples tested. The results of studies of calf sturgeon revealed the presence of a number of microorganisms: Escherihia coli, Proteus vulgaris, Escherihia coli haemolitica, Staphylococcus haemoliticus, molds of the genus Aspergillus in different percentages. When determining the sensitivity of isolated cultures to antibacterial drugs by the disc diffusion method, it has been established that Staphylococcus and Escherihia coli haemolitica cultures are susceptible to all antibiotics; Proteus vulgaris exhibits resistance to ampicillin, amoxicillin.

Microsporіа affect different species of animals and humans. The high contagiousness of the pathogen determines the relevance of research into this disease. Microsporum canis is the pathogen that most often causes microsporia. Weakened functions of the immune system and violation of the epithelial barrier of the skin are a favourable factor that causes microspores. The main source of infection is cats, which are involved in the storage and transmission of the pathogen. To clarify the dynamics of morphological, immunological and histological changes in microsporia, blood and skin studies of guinea pigs infected with M. canis were carried out. The animals were divided into two groups of 6 guinea pigs (healthy and sick). Test material (blood and skin) was taken from clinically healthy and sick animals 21 and 42 days after infection. The number of erythrocytes and leukocytes was determined by counting them in the Goryaev chamber, the hemoglobin content – by the method of cyanide hemoglobin. The leukogram was derived based on the counting and differentiation of 200 leukocyte cells in blood smears. Material for histological examination (pieces of skin) was fixed in 10–12% cooled solution of neutral formalin, followed by pouring in paraffin according to the scheme proposed by G. A. Merkulov. The obtained results demonstrated that leukocytosis developed in guinea pigs with microsporia on the 21st and 42nd days; the number of rod-shaped neutrophils increased, that of segmental neutrophils decreased, and that of ESR increased. The immune response to the course of microsporia was manifested in an increase in the percentage of T-lymphocytes, T-suppressors and a decrease in T-helper cells and an increase in T-killers compared with healthy animals. Histological examination showed that on the 21st day after infection, hyphae and spores of the fungus M. canis were localized in the skin. There is swelling of the dermis, stratification of collagen fibers and the accumulation of inflammatory infiltrates around the hair follicles. On the 42nd day, the infiltration spread and dystrophic changes in the skin occurred in the form of desquamation of the epidermis and the formation of acanthosis and hyperkeratosis on the surface of the dermis. The conducted research will allow further assessment of the course of microsporia under the action of various drugs and help establish the most effective method of treatment.

Microsporia is the most common disease of fungal etiology, most often caused by the pathogen Microsporum canis. Treatment of this disease requires a comprehensive approach, because the disease is dangerous to humans. Therefore, in the treatment of microspores, it is important not only to carry out therapeutic measures, but also to prevent the spread of the pathogen of the fungus in the environment and increase the immune status of the organism in the fight against infection. The main source of infection is cats. In order to determine the effectiveness of treatment of microsporia by various methods, studied of the blood and skin of guinea pigs infected with the pathogen M. canis. Sick animals were divided into three groups. The first group was treated with the systemic antifungal itraconazole and topical treatment with a solution of clotrimazole. Treatment of the second group was performed with a topical antifungal agent (1% solution of clotrimazole) with vaccination with the antifungal vaccine “Vakderm”. For the third group, the developed drugs were used – antifungal agent “Micromar” and immunostimulant “Biogluk”. During treatment, hematological and immunological blood researches and histological skin examinations were performed. During treatment with antifungal drugs (itraconazole and clotrimazole) the number of leukocytes decreases from 11.13 ± 0.72 to 7.13 ± 0.22, rod-shaped neutrophils from 15.76 ± 1.29 to 5.50 ± 0.76, and segmental increases from 12.17 ± 1.47 to 24.17 ± 2.27, decreases ESR from 5.67 ± 0.67 to 2.33 ± 0.42, which occurs when inhibiting the inflammatory response of the organism to infection. Thrombocytopenia (from 231.17 ± 7.60 to 184.33 ± 7.65) and eosinophilia (from 2.70 ± 0.73 to 7.33 ± 1.33) are also noted. There is a slight increase in T-helpers and a decrease in T-suppressors. Histologically, the infiltration of the dermis by histiocytes and  eosinophils under skin  persists. In the treatment of microsporia by treatment with 1 % solution of clotrimazole and vaccination with the vaccine “Vakderm” the results of studies showed that the number of leukocytes decreases from 11.13 ± 0.72 to 5.35 ± 0.31 (P < 0.01), rod-shaped neutrophils from 15.76 ± 1.29 to 7.67 ± 0.56, and segmental increases from 12.17 ± 1.47 to 22.17 ± 0.91 (P < 0.001), decreases). The number of T-helpers is increasing. The histological picture on day 7 is characterized by hyperkeratosis, and on day 14 the hyperemia of the basal layer of the epidermis persists. When using the antifungal drug “Micromar” and immunostimulant “Biogluk” the results of studies showed that the number of leukocytes decreases from 11.13 ± 0.72 to 6.95 ± 0.10, rod-shaped neutrophils from 15.76 ± 1.29 to 6.17 ± 0.65, and segmental increases from 12.17 ± 1.47 to 22.00 ± 0.86, decreases ESR from 5.67 ± 0.67 to 2.17 ± 0.31. Increases the number of T-helpers and the number of natural killers and T-suppressors is gradually decreasing. Histological changes are presented in the form of dilation of blood vessels and visualization of single erythrocytes in the dermis on day 14 of treatment.

National biosecurity is a system of organizational and technical measures that help protect humans, animals and the environment from potential and actual biological threats. That is why analysis of the main hazardous biological sources for humans and animals has been carried out. The influence of climate change on the animals’ welfare as well as their predisposition to the deferred type tendency is proved. The prevalence of vector diseases of animals, mycoses and mycotoxicoses, which can manifest themselves in the form of such emergencies as outbreaks of exotic diseases, large-scale epizootics, a sharp increase of the incidence of endemic diseases, etc., are of both ecological and biological danger. Contagious vesicular (nodular) dermatitis (Lumpy skin disease, LSD) is caused by the virus belonging to the genus Capripoxvirus, the Poxviridae family and affects mainly cattle and buffaloes. The virus is distributed mainly by means of mechanical carriers (species of Stomoxys spp. and other flies). Global warming on the European continent facilitates the migration of blood-sucking insects that are carriers of vector diseases. As a result, the insects occupy new ecological niches adapting to the new biocenoses. A poorly controlled movement of large numbers of livestock also creates a risk of disease spreading. The World Organization for Animal Health (OIE) has identified LSD as highly dangerous illnesses subject to notification. The focus is made on policies dealing with the control and eradication of contagious nodular dermatitis in case of its detection. The spread of LSD can be prevented through the introduction of biosafety measures at the farm level and the introduction of restrictions on the movement of infection-susceptible animals and goods from the infected territories. Vaccination is the most effective disease control tool in endemic areas. LSD control and elimination policy in case of disease detection includes sanitary slaughter (stamping-out) of infected animals – a complex of antiepizootic measures that carry out by direction  of the chief state inspector, including the slaughter of sick and infected animals of the herd, and if necessary, animals of another herd that could lead before the transmission of a pathogenic agent – the pathogen of contagious nodular dermatitis of cattle. All animals, that are susceptible to the disease, whether vaccinated or not, are clogged and their carcasses are destroyed by burning, burial with the guarantee of preventing the spread of infection through carcasses or other products of dead animals. Emergency control of outbreaks envisages the ring vaccination of buffer zones within 25–50 km from infected areas, as well as foundation of temporary or permanent slaughter places in the infected areas. Sufficient herd immunity must be created and maintained within large territories both around the infected area and at the borders with infected countries. Such immunity is achieved when 80% of the herd is covered by vaccination.

The most common fungal skin disease in animals is microsporia; the principal causative agent is the fungus Microsporum canis. The disease is of fungal etiology and highly contagious. The pathogen can stay in the environment for a long time and pose a risk of human infection. Cats, especially those walking on the street, are a significant factor in transmitting the infection. Spores of Microsporum canis can persist on the surface of the cat's body for a long time and, under favorable conditions, can provoke clinical manifestations of the disease. Under these circumstances, they are intensively distributed in the environment. Therefore, choosing the optimal therapeutic approach to solving this problem is essential. Treatment of sick animals should be practical and aimed at preventing the spread of the pathogen and cost-effective and beneficial to both veterinary specialists and owners of sick cats, as the duration of treatment is on average 14–21 days in order to determine the cost-effectiveness of treatment of microsporia in cats with the antifungal agent “Micromar” and immunostimulant “Biogluk” in comparison with other schemes, the calculation of the cost-effectiveness ratio CER. This made it possible to obtain accurate and complete data, as it took into account the cost of treatment and the number of animals that recovered during the complex therapy. Sick animals were divided into three groups. Each sick animal received treatment for 21 days. The first group was treated with the systemic antifungal itraconazole and treated with a 1 % solution of clotrimazole. The second group was vaccinated twice with Vakderm antifungal vaccine and treated daily with a topical antifungal agent (1 % clotrimazole solution). The third group used the antifungal agent “Micromar” and immunostimulant “Biogluk”. To control the quality of therapy, culture was performed on the nutrient medium for dermatophytes from the affected areas of the animal's body. The calculation of cost-effectiveness indicators showed that the treatment of cats with microsporia is effective in all groups of studied animals, as recovery occurred in each patient. However, the costs of therapy are different. In particular, in the first group, the CER coefficient is – 48.00 hryvnias, in the second – 42.00 hryvnias, in the third – 13.00 hryvnias.