Introduction. According to the World Health Organization, the number of patients with diseases of the nervous system in the world is increasing every year. The use of phytopreparations is a promising direction of pharmacotherapy; preparations from medicinal plant materials have a number of advantages in the treatment of chronic diseases.Aim. Qualitative analysis and quantitative determination of the main groups of biologically active substances collected from medicinal plant materials.Materials and methods. As an object of study, we used a collection of medicinal plant materials developed at the Department of Pharmaceutical Technology of the PSFA together with the Department of Pharmacognosy. Quantitative determination of biologically active substances of the collection was carried out by spectrophotometric method [Jenway PortLab 511 UV spectrophotometer PortLab Int., UK)] and by visual titration.Results and discussion. In this work, a qualitative analysis and quantitative determination of the content of flavonoids, phenolic compounds, sesquiterpenic acids and ascorbic acid was carried out, and the water absorption coefficient of the collection was determined.Conclusion. As a result of the work, the content of flavonoids, phenolic compounds, sesquiterpenic acids and ascorbic acid in the developed collection was confirmed, their amount was determined, which directly affects the pharmacological effect of the collection.

Introduction. Strawberry garden leaf extract dry (strawberry extract) is one of the potential substances for obtaining safe medicines for the treatment of diseases of the urinary tract, as it has diuretic and anti–inflammatory activity. The study of chronic toxicity is a mandatory step in the preclinical assessment of the safety of a new pharmacological substance.Aim. Preclinical studies of the chronic toxicity of the substance – strawberry extract.Materials and methods. The object of the study was strawberry extract obtained at JSC "Biohimmash". The study of the chronic toxicity of strawberry extract with prolonged administration to rats was carried out in the Laboratory of Medicinal Toxicology of the All-Russian Research Institute of Medicinal and Aromatic Plants in accordance with the "Guidelines for Experimental (Preclinical) Study of new pharmacological Substances" [1]. Laboratory animals were divided into three groups of 15 rats each: group I – control (water); group II – strawberry extract at a dose of 72 mg/kg; group III – strawberry extract – 720 mg/kg.Results and discussion. As a result of a 3-month experiment, it was found that strawberry extract with intragastric administration does not cause the death of animals and does not have a general toxic effect. Also, strawberry extract with intragastric application shows a stable diuretic effect and its preservation during a three-month experiment.Conclusion. Thus, the chronic toxicity of strawberry extract was studied. It was found that strawberry extract does not have a general toxic effect. The conducted research shows the prospects for further research on the development and production of medicines from strawberry leaves with dry extract, which have diuretic and anti-inflammatory activity.

Abstract. The growing demand for medicinal herbal preparations, the changing requirements of the regulatory system for their effectiveness and safety during the registration procedure pose certain tasks for their study. For the prevention and treatment of various diseases, along with a wide range of synthetic medicines, herbal medicines are used. At the same time, not only medicinal plants and collections are used as herbal medicines, but also various plant extracts, individual biologically active compounds isolated from plants. A preclinical study of reproductive toxicity seems necessary, since it is known that some medicinal plants have a teratogenic effect [1]. The main task of the preclinical study of the effect of potential drugs on the reproductive system is to prove or exclude the possibility of developing a teratogenic effect caused by a new pharmacological agent in an animal experiment. Mandatory testing for reproductive toxicity should be subject to new, original pharmacological agents, as well as well-known drugs recommended for use in long repeated courses, as well as for the treatment of pregnant women and when prescribed during lactation; as preventive agents and contraceptives [2, 3]. Strawberry garden leaf extract dry (strawberry garden extract) is one of the potential substances for obtaining safe medicines for the prevention and treatment of diseases of the urinary tract, as it has diuretic and anti–inflammatory activity [4]. The study of the reproductive toxicity of strawberry garden extract is a mandatory part of the preclinical safety assessment of a new pharmacological substance for obtaining safe medicines with it. In this regard, studies on the reproductive toxicity of strawberry garden leaves of dry extract are relevant in the framework of preclinical studies and substantiation of the use of the substance for the further development of medicines based on it.