Quality assessment of chrysanthemum indicum flower by simultaneous quantification of six major ingredients using a single reference standard combined with HPLC fingerprint analysis, Asian Journal of Pharmaceutical Sciences (2015), http://dx.doi.org/ABSTRACT Chrysanthemum Indicum Flower is usually consumed as functional food. This paper described an improved total quality assessment method for Chrysanthemum Indicum Flower by simultaneous quantitation using a single standard to determine multi-components method combined with high performance liquid chromatography fingerprint analysis. Six main components of Chrysanthemum Indicum Flower including two flavonoids and four phenolic acids were simultaneously quantified using linarin as the internal reference standard. The method was fully validated with respect to linearity, precision, accuracy, robustness and stability. The validated method was successfully applied to the analysis of thirty three batches of Chrysanthemum Indicum Flower samples. Under the same chromatographic conditions, fingerprint analysis in combination with Similarity analysis and principal component analysis was performed to identify the samples from different regions. In general, an effective assessment using a single standard to determinate multi-components method combined with fingerprint analysis make the reliable qualitation and quantitation analysis of Chrysanthemum Indicum Flower available.
A simple and reliable method using high-performance liquid chromatography coupled with a photodiode array detector (HPLC-PDA) was firstly established for the determinations of eleven bioactive compounds (neomangiferin, mangiferin, spinosin, liquiritin apioside, liquiritin, fumalic acid, 6'''-feruloylspinosin, senkyunolide I, isoliquiritin, glycyrrhizic acid and senkyunolide A) in Suanzaoren decoction (SZRD) extract and its granules. The chromatographic analysis was performed on a C18 column at 30°C. Excellent linear behaviors over the investigated concentration ranges were observed with the values of R(2) being higher than 0.9990 for all analytes. The developed method showed good precision and accuracy with overall intra- and inter-day variations of less than 2.0%, and overall recoveries in the range of 97.2 - 102.1%. The validated method was successfully applied to the determination of eleven components in SZRD samples from different production batches, including SZRD extract, lab-made SZRD granules and clinical medicine. This accurate and reliable HPLC-PDA method will be helpful for improving the quality evaluation of SZRD granules and its quality control in productive processes.
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