Alterations of cellular redox status are closely associated with physiological and pathological processes.Glutathione (GSH) and H 2 O 2 should be the most representative redox couple in living cells. However, up to now, there is no way to reversibly detect GSH/H 2 O 2 . In this report, a near-infrared (NIR) fluorescent probe (Cy-O-Eb) for monitoring the changes of GSH/H 2 O 2 levels in vivo was developed based on switching on-off a five-membered ring involved in ebselen. This probe could reversibly respond to GSH and H 2 O 2 with high selectivity, sensitivity and mitochondrial targeting. It was successfully used to monitor the changes of redox status during apoptosis and the H 2 O 2 changes at the wound margin in zebrafish larvae. Thus, the probe would provide an ideal tool for monitoring redox status changes and studying molecular events involved in redox regulation.

No abstract

A novel fluorescence probe (HB) has been designed and synthesized to image selenol in living cells and in vivo for the first time, and used to investigate the Na2SeO3 anticancer mechanism in HepG2 cells.