By experiments, separate and purify efficient sulfate—reducing bacteria (SRB), which is identified as Desulfobacter. With its treatment of acid mine drainage(AMD) containing Fe2+ and Mn2+, obtain the optimum conditions that pH=6,temperature=37,vibrating intensity=100r/min, COD/SO42- =2.Under this condition ,the removal rate of SO42-、Fe2+ and Mn2+ is 88.16%、99.37% and 59.18% respectively with the initial concentration 1411mg/L for SO42-,257mg/L for Fe2+ and 325mg/L for Mn2+.This puts a theoretical foundation for the further study.

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