Anthocyanins have important biological functions, it can help plants resist diseases invasion and pest attack. In this study, we got Rosea1-transformed snapdragon by Agrobacterium-mediated method. Anthocyanins content in the transgenic snapdragon was significantly elevated. Compared with wild-type plant, the snapdragon with anthocyanins accumulation in plant effectively reduced aphid attack in farmland. Similarly, Over-expression of Rosea1 in heterologous plant tobacco also lead to anthocyanins synthesis and the transgenic tobacco showed resistance to aphids. Therefore, improving the content of anthocyanins in plants using genetic engineering technology is a useful measure for plant protection.

High temperature was an restrictive factor for tree peony growth and distribution in the world. In this study, we identified a heat shock transcriptor PsHsfB1 from our tree peony gene database. Further analysis revealed that the PsHsfB1 was sequence conserved and closely to Hsfs in others plant species. Gene Ontology annotation revealed that the PsHsfB1 was correlated with theromotolorance of tree peony.

Heat shock proteins (Hsp) were usually regarded as chaperones and play important role in plant stress tolerance. The expression of Hsps would be inducted when plant encounter lots of environmental stressors. Lactuca sativa was an important leaf vegetable in the world, but thermol stress seriously restricted its production. In this study, we isolated the Hsp70 members from Lactuca sativa database, a total of 5 Hsp70 members with completed open reading frame (ORF) were selected and further analysized. Results showed that the 5 LsHsp70 members were closely to Hsp70s in Arabidopsis, which indicated that the identified Hsp70 may responsible for thermotolerance in Lactuca sativa. The 5 new Hsp70s would be used as candidated genes for improving the tolerance trait of Lactuca sativa and others plants.

In this paper, an activator protein was isolated and identified from crude protein of Botrytis Spp which was separated from the soil of the wheat field with the method of mycelium recycling, breaking and centrifuging,etc. The activator protein appeared a single band on SDS-PAGE whose molecular weight was about 42kD. At the same time, it had a preliminary activity test and investigated on this activator protein. This study indicated that this activator protein is able to promote wheat germination after the deal with it, especially when the density is 3μg/mL, the seed germination is the best. So it is proved that the plant activator protein has really the biological function of improving the wheat germination.

Anthocyanin mainly responsible for flowers color in many plant species, it also accumulated in response to lots of environmental stress to reduce the damage to plant cell. Anthocyanin synthesis (ANS) protein is an important synthetase participated in anthocyanin biosynthetic pathway. In this study, we isolated the PsANS gene from transcriptome database built by our previous study. The PsANS gene contain an 1050bp open reading frame encoding 349 amino acid, phylogenetic analysis revealed that PsANS was segrated into a group with ANS from others plant species. Secondary and thri-dimension structure prediction also revealed that it may have similar function with ANS in others plant species. The identified PsANS gene would be helpful for further research in flower color modification and resistance breeding.