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This study was carried out to determine direct and indirect effects of some characters (Days to 50% flowering, plant height (cm), Number of monopodial branches per plant, Number of Sympodia per plant, Number of bolls per plant, Mean boll weight (g), Ginning percentage, Seed index (g) and Lint index (g)) on seed cotton yield. The experiment was carried out with 48 F 1 hybrids have produced through Line×Tester mating design using 6 hirsutum non Bt lines (RAH 318, RAH 243, RAH 128, RAH 146, RAH 97 and RAH 124) and 8 hirsutum non Bt testers ( SC 14, SC 18, SC 7, SC 68, RGR 32, RGR 24, RGR 58 and RGR 37)were sown in a randomized complete block design (RCBD) with two replications at the Agricultural Research Station, Bavikere, UAS, Bangalore.