Nanoparticles consisting of a derivative of the blue-emitting conjugated polymer polyfluorene doped with green-, yellow-, and red-emitting conjugated polymers were prepared by a reprecipitation method. The nanoparticles can be described as a system of densely packed chromophores that exhibit efficient energy transfer from the host to the dopant polymers. Fluorescence quenching analysis of the host polymer as a function of the dopant concentration indicates that one energy acceptor molecule can effectively quench 90% of the fluorescence of a nanoparticle consisting of 100-200 host conjugated polymer molecules. A nanoparticle energy transfer model was developed that successfully describes the quenching behavior of a small number of highly efficient energy acceptors per nanoparticle. The fluorescence brightness of the blended polymer nanoparticles was determined to be much higher than that of inorganic quantum dots and dye-loaded silica particles of similar dimensions. The combination of high fluorescence brightness and tunable fluorescence of these blended nanoparticles is promising for ultrasensitive fluorescence-based assays.

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We report the results from a new pump/probe spectrometer for potential use in combustion diagnostics that employs asynchronous optical sampling. The instrument consists of two frequency-doubled mode-locked Nd:YAG lasers operating at slightly different repetition rates, synchronously pumping two dye lasers (rhodamine 6G) to generate the pump and probe beams. The spectral and temporal capabilities of the instrument are examined by obtaining a spectrum and an excited state decay of rhodamine B. The instrument response is shown to be proportional to pump power, probe power, and sample absorptance. Different frequency synthesizers and different modes of triggering are used to study their effect on signal stability.

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