Recently, drug discovery has focused on developing targeted therapy methods, which requires identifying the exact cellular processes and molecular interactions. Peptides are highly functional molecules that can be tailored to achieve desirable properties which make them an attractive therapeutic agent. In this study, we applied and developed methods to engineer constrained peptides to recognise a special DNA structure, called G-quadruplex (G4).G-quadruplexes are four-stranded non-canonical nucleic acids secondary structures that are found in human genome and their formation and stabilization is linked to genome instability and cancer. Thus, these unique nucleic acids structures have become attractive targets for therapeutic design of molecules. In the past decades, several classes of compounds have been developed to stabilized and target G4s. However, despite the