Precancerous lesions of the liver are the intermediate stage in the development of liver cancer from cirrhosis. Our previous studies indicated that Shanxian granules (SXG) can alleviate abnormal proliferation of liver cells and the formation of cirrhosis. To explore the mechanism of SXG-containing serum in reversing epithelial-mesenchymal transition (EMT) and malignant transformation of hepatic oval cells (HOC). The malignant transformation and EMT of rat HOC cell line WB-F344 were induced by N-methyl-N’-nitro-N-nitroso (MNNG ). Subsequently, WB-F344 cells treated with MNNG were treated with with three doses of SXG to observe its inhibitory effect. The Wnt signaling pathway was blocked by adding Wnt activator CP21R7 on the basis of SXG high dose group to observe whether the inhibitory effect was regulated by Wnt/β-catenin signaling pathway.SXG-containing serum inhibited MNNG-stimulated proliferation, migration and invasion of WB-F344 cells in a time-and dose-dependent manner. In MNNG-stimulated WB-F344 cells, SXG-containing serum regulated the expression of tumor-related indicators and EMT-related proteins、Wnt signaling pathway-related proteins.The results showed that SXG-containing serum reversed WB-F344 EMT and malignant transformation through Wnt/β-catenin signaling pathway, which provides a scientific basis for the clinical application of SXG .