Aims: To determine if there is a moderating effect of cultural intelligence between the conflict of nursing relationships and teamwork.Background: Relationship conflicts that exist in the nursing teamwork cannot be ignored. It would lead to poor teamwork and impact on patients' poor outcomes. In the meantime, cultural intelligence has been proven that people who have a higher cultural intelligence can do better at the work. Methods:The sample included 550 clinic nurses from five different hospitals in Shanghai. The Cultural Intelligence Scale, the Relationship Conflict Scale, and the Nursing Teamwork Survey Scale were used to investigate. Through the Johnson-Neyman method and SPSS Process Macro, the moderating effect of cultural intelligence was analysed. Results:The findings showed that cultural intelligence was founded to be significant (b = 2.03, p < 0.01). When the cultural intelligence score is greater than or equal to 90.8, the relationship conflict between nurses will not affect teamwork. Conclusions:The findings of this study may provide data to improve nurses' performance in team cooperation.