The aim of the present study was to observe the effect of Spirulina supplementation on DNA damage in mice blood cell and blood redox status after acute exercise. 40 male Kunming mice was randomly assigned into four groups: Control group (CG), exercise group without Spirulina supplementation (EG), Spirulina supplementation group without exercise (SG) and Spirulina supplementation group with exercise (SEG). The exercise fatigue model was built up for mice through the protocol of repeated exhaustive treadmill running for seven days and the single cell gel electrophoresis (SCGE) was used to detect the DNA damage of blood cells in different groups. Blood samples were drawn from different groups and the changes of SOD and GSH which stand for anti-oxidative level in plasma. Also, Malondialdehyde (MDA), a marker of lipid peroxidation, was measured in the plasma of mice.This studies showed that the DNA damage of blood cell in SEG group was significantly lower than that of EG group (P<0.01) and there was no statical difference between CG and SG groups. And the level of GSH in SEG group were significantly lower than that of EG group (P<0.01).This results showed that plasma concentrations of malondialdehyde (MDA) were significantly decreased after supplementation with spirulina (P < 0.001). Those results leaded to conclusion that acute exercise induced more free radical to change the level of SOD, GSH and MDA and also occurred more DNA damage. It was suggested that DNA breakage occurs in blood cell after exhaustive exercise as a consequence of oxidative stress and the Spirulina supplementation has obvious protective effect to prevent exercise-induced DNA damage.

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