Human immunodeficiency virus (HIV)-1 is mainly transmitted mucosally during sexual intercourse. We therefore evaluated the protective efficacy of a vaccine active at mucosal sites. Macaca mulatta monkeys were immunized via both the intramuscular and intranasal routes with an HIV-1 vaccine made of gp41-subunit antigens grafted on virosomes, a safe delivery carrier approved in humans with self-adjuvant properties. Six months after 13 vaginal challenges with simian-HIV (SHIV)-SF162P3, four out of five vaccinated animals remained virus-negative, and the fifth was only transiently infected. None of the five animals seroconverted to p27gag-SIV. In contrast, all 6 placebo-vaccinated animals became infected and seroconverted. All protected animals showed gp41-specific vaginal IgAs with HIV-1 transcytosis-blocking properties and vaginal IgGs with neutralizing and/or antibody-dependent cellular-cytotoxicity activities. In contrast, plasma IgGs totally lacked virus-neutralizing activity. The protection observed challenges the paradigm whereby circulating antiviral antibodies are required for protection against HIV-1 infection and may serve in designing a human vaccine against HIV-1-AIDS.

The question of whether or not Ginkgo biloba still exists in the wild has been debated by botanists, without resolution, for almost a hundred years. Most of the controversy has focused on a single population of trees located on Tian Mu Shan (Tian Mu Mountain) in Zhejiang Province, China, a site of human activities for approximately 1500 years. Regardless of its origin, the Tian Mu Shan Ginkgo population is biologically significant by virtue of its long survival in a semi‐natural state under conditions of intense interspecific competition. A total of 167 Ginkgos were counted and measured in the 1018 ha Tian Mu Shan Reserve. Many of the trees were growing on disturbance‐generated microsites, such as stream banks, steep rocky slopes, and the edges of exposed cliffs. Forty percent of the censused individuals were multitrunked, consisting of at least two trunks greater than 10 cm in diameter at breast height. Most of these secondary trunks originated from root‐like “basal chichi,” that are produced at the base of trees that have experienced damage from soil erosion or other factors. No Ginkgos less than 5 cm in basal diameter were found in the mature forests of Tian Mu Shan. This lack of seedling reproduction is caused by several factors: (1) the lack of sunny microsites suitable for seedling growth, (2) seed collection by people, and (3) seed predation by animals. In the absence of successful seedling establishment, secondary trunk formation from basal chichi is the single most important factor in explaining the long term persistence of Ginkgo on Tian Mu Shan.

Land use land cover (LULC) transition analysis is a systematic approach that helps in understanding physical and human involvement in the natural environment and sustainable development. The study of the spatiotemporal shifting pattern of LULC, the simulation of future scenarios and the intensity analysis at the interval, category and transition levels provide a comprehensive prospect to determine current and future development scenarios. In this study, we used multitemporal remote sensing data from 1980–2020 with a 10-year interval, explanatory variables (Digital Elevation Model (DEM), slope, population, GDP, distance from roads, distance from the city center and distance from streams) and an integrated CA-ANN approach within the MOLUSCE plugin of QGIS to model the spatiotemporal change transition potential and future LULC simulation in the Greater Bay Area. The results indicate that physical and socioeconomic driving factors have significant impacts on the landscape patterns. Over the last four decades, the study area experienced rapid urban expansion (4.75% to 14.75%), resulting in the loss of forest (53.49% to 50.57%), cropland (21.85% to 16.04%) and grassland (13.89% to 12.05%). The projected results (2030–2050) also endorse the increasing trend in built-up area, forest, and water at the cost of substantial amounts of cropland and grassland.

Understanding the driving factors and assessing the risk of rainstorm waterlogging are crucial in the sustainable development of urban agglomerations. Few studies have focused on rainstorm waterlogging at the scale of urban agglomeration areas. We used the Guangdong-Hong Kong-Macao Greater Bay Area (GBA) of China as a case study. Kernel density estimation (KDE) and spatial autocorrelation analysis were applied to study the spatial distribution characteristics of rainstorm waterlogging spots during 2013–2017. A geographical detector (GD) and geographically weighted regression (GWR) were used to discuss the driving mechanism of rainstorm waterlogging by considering eight driving factors: impervious surface ratio (ISR), mean shape index of impervious surface (Shape_MN), aggregation index of impervious surface (AI), fractional vegetation cover (FVC), elevation, slope, river density, and river distance. The risk of rainstorm waterlogging was assessed using GWR based on principal component analysis (PCA). The results show that the spatial distribution of rainstorm waterlogging in the GBA has the characteristics of multicenter clustering. Land cover characteristic factors are the most important factors influencing rainstorm waterlogging in the GBA and most of the cities within the GBA. The rainstorm waterlogging density increases when ISR, Shape_MN, and AI increase, while it decreases when FVC, elevation, slope, and river distance increase. There is no obvious change rule between rainstorm waterlogging and river density. All of the driving factors enhance the impacts on rainstorm waterlogging through their interactions. The relationships between rainstorm waterlogging and the driving factors have obvious spatial differences because of the differences in the dominant factors affecting rainstorm waterlogging in different spatial positions. Furthermore, the result of the risk assessment of rainstorm waterlogging indicates that the southwest area of Guangzhou and the central area of Shenzhen have the highest risks of rainstorm waterlogging in GBA. These results may provide references for rainstorm waterlogging mitigation through urban renewal planning in urban agglomeration areas.

No abstract